乳腺癌组织HER2基因扩增的检测方法
| 作者: |
1王晓舟,
2翟云良,
2杨振,
2倪晓龙,
2孙尧,
3李世宝
1 徐州医科大学基础医学院，江苏 徐州 221000 2 苏州天隆生物科技有限公司，江苏 苏州 215123 3 徐州医科大学附属医院检验科，江苏 徐州 221000 |
| 通讯: |
王晓舟
Email: sdjnshlb@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.09.022 |
| 基金: | 江苏省青年医学人才, QNRC2016781 |
摘要
目的：评价高分辨率熔体聚合酶链反应(high-resolution melt polymerase chain reaction，HRM-PCR)检测HER2基因扩增的有效性及其与荧光原位杂交(fluorescence in situ hybridization，FISH)和免疫组织化学(immunohistochemistry，IHC)检测法的一致性。方法：采用HRM-PCR法检测HER2阴性及阳性细胞株，评估检测方法的有效性；检测98例已行FISH和/或IHC的临床样本，并与FISH和IHC结果进行比较。结果：HRM-PCR检测可以有效区分HER2阴性及阳性细胞株(P<0.05)，具有较好的可重复性；检测98例临床样本显示，阴性和阳性样本检测结果差异有统计学意义(0.18±0.14 vs 1.42±0.88，P<0.01)，与IHC的一致性为80.33%(kappa=0.6，P<0.01)，与FISH的一致性为87.88%(kappa=0.7，P<0.01)，结论：HRM-PCR是一种可靠有效的检测HER2基因扩增的方法，与FISH和IHC均有较好的一致性。
关键词：
HER2基因
HRM-PCR
乳腺癌
荧光原位杂交
免疫组织化学
Detecting methods for HER2 gene amplification in breast cancer tissue
CorrespondingAuthor: WANG Xiaozhou Email: sdjnshlb@126.com
DOI: 10.3978/j.issn.2095-6959.2017.09.022
Abstract
Objective: To evaluate the detection of HER2 gene amplification effectiveness by high-resolution melt polymerase chain reaction (HRM-PCR) and compare with fluorescence in situ hybridization (FISH) and/or immunohistochemistry (IHC). Methods: HRM-PCR was used to test 98 clinical samples which has detected by FISH and/or IHC, and the results of FISH and IHC were compared. Results: HER2 negative and positive cell lines can be distinguished by HRM-PCR (P<0.05) with well repeatability. The difference between negative and positive samples results was significant (0.18±0.14 vs 1.42±0.88, P<0.01), and 80.33% consistency with IHC (kappa =0.6, P<0.01), 87.88% with FISH (kappa =0.7, P<0.01). Conclusion: HRM-PCR is a reliable and effective method to detect HER2 gene amplification which has a high consistency with FISH/IHC.
Keywords：
HER2 gene
HRM-PCR
breast cancer
fluorescence in situ hybridization
immunohistochemistry