Objective: To investigate the expression of Stanniocalcin 1 ( STC-1) in cervical cancer and its relation with prognosis. Methods: STC-1 protein expression in cervical cancer cell lines was determined by Western blot. We examined STC-1 expression in human cervical cancer tissues by immunohistochemical staining (IHC), and investigated the clinical correlation between STC-1 tissue levels and the clinicopathological characteristics of cervical cancer patients. Kaplan-Meier curves for survival in cervical cancer patients were determined based on the analysis of STC-1 expression from online database. Results: STC-1 was markedly overexpression in all cervical cancer cell lines compared with normal cells. IHC results revealed that STC-1 was mainly expressed in the cell membrane and plasma. STC-1 protein levels were significantly higher in cervical cancer tissues when compared with matched non-cancerous tissues (P<0.05), and associated with lymph node metastasis (P=0.038). Kaplan-Meier curves for survival analysis of online database showed that patients with high STC-1 expression had poor prognosis (P<0.01). Conclusion: STC-1 expression is correlated with lymph node metastasis of cervical cancer patient and associated with prognosis, may be a potential bio-marker for prognosis.