非小细胞肺癌患者胸腔积液中EGFR基因突变的检测及临床意义
| 作者: |
1毛旭华,
1汤俊明,
1乔国洪,
1陈霞
1 江苏大学附属宜兴市人民医院检验科，江苏 无锡 214200 |
| 通讯: |
陈霞
Email: staff1291@yxph.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.08.006 |
| 基金: | 无锡市卫计委面上项目, MS201536 |
摘要
目的:探讨采用非小细胞肺癌患者胸腔积液标本肿瘤细胞进行表皮生长因子(epidermal growth factor receptor，EGFR)基因突变检测的可行性及其临床意义。方法:采用Sanger测序法检测17例 非小细胞肺癌患者胸腔积液及对应的17例手术或肺部穿刺组织标本EGFR基因18~21外显子基因突 变，并进行统计分析。结果:胸腔积液标本17例共检出5例突变，检出率29.41%。手术或穿刺组织 标本17例共检出7例突变，检出率41.18%。胸腔积液标本EGFR基因突变检出率略低于手术或穿刺 组织标本。结论:采用Sanger测序法进行非小细胞肺癌患者胸腔积液中EGFR基因突变的检测，方 法可行，尤其适用于无法获取手术或肺部穿刺组织标本的患者。
关键词：
非小细胞肺癌
胸腔积液
EGFR基因
Detection of EGFR mutations and its clinical signi cance in hydrothorax of patients with non-small cell lung cancer
CorrespondingAuthor: CHEN Xia Email: staff1291@yxph.com
DOI: 10.3978/j.issn.2095-6959.2017.08.006
Abstract
Objective: To explore the clinical signi cance of detecting epidermal growth factor receptor (EGFR) mutations in hydrothorax of patients with non-small cell lung cancer (NSCLC). Methods: e mutations of EGFR in exon 18– 21 were detected with Sanger sequencing in 17 hydrothorax samples and 17 tumor tissue samples in parallel from patients with NSCLC. Results: ere were 5 cases (29.41%) with EGFR mutations in 17 hydrothorax samples and 7 cases (41.18%) in 17 tumor tissue samples. e detection rate of EGFR mutations in hydrothorax samples was lower than that in tumor tissue samples. Conclusion: e hydrothorax sample can be used to detecting EGFR mutations by Sanger sequencing. And it is helpful to patients with NSCLC who can’t obtain tumor tissue.
Keywords：
non-small cell lung cancer
hydrothorax
Epidermal growth factor receptor