142例结外NK/T细胞淋巴瘤免疫组织化学分析
| 作者: |
1何同梅,
1许春伟,
1陈燕坪
1 福建医科大学附属福建省肿瘤医院病理科，福州 350014 |
| 通讯: |
陈燕坪
Email: kelf2006@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.06.011 |
| 基金: | 国家临床重点专科建设项目, 2013 福建省卫生厅青年创新课题, 2014-1-15 |
摘要
目的：探讨结外NK/T细胞淋巴瘤(extranodal NK/T-cell lymphoma，ENKTL)的免疫组织化学特征。方法：回顾性分析142例ENKTL的免疫组织化学结果及EBER原位杂交结果，并用PCR方法检测1例ENKTL的TCR基因重排。结果：ENKTL免疫组织化学阳性率CD2为87.9%(116/132)，CD3为99.3%(141/142)，CD5为33.3%(44/132)，CD7为74.7%(74/99)，CD4为30.2%(13/43)，CD8为35.9%(14/39)，CD56为92.9%(132/142)，TIA-1为100%(132/132)，颗粒酶B为99%(111/112)，穿孔素为100%(6/6)，CD30为40.9%(36/88)，PDGFRA为51.9%(28/54)，CMYC为53.7%(29/54)，Ki-67指数50%~90%，中位数为80%。EBER原位杂交阳性率为100%(142/142)。TCR基因重排结果阴性。结论：结外NK/T细胞淋巴瘤存在较为特征性的免疫组织化学表型，并且EBER原位杂交呈阳性。
关键词：
结外NK/T细胞淋巴瘤
免疫组织化学
原位杂交
Immunohistochemical analysis of 142 cases of extranodal NK/T-cell lymphoma
CorrespondingAuthor: CHEN Yanping Email: kelf2006@126.com
DOI: 10.3978/j.issn.2095-6959.2017.06.011
Abstract
Objective: To investigate the immunohistochemical features of extranodal NK/T-cell lymphoma (ENKTL). Methods: A retrospective analysis of immunohistochemical results of 142 cases of ENKTL was performed. In situ hybridization was performed to detect the presence of EBV encoded small RNA (EBER), and polymerase chain reaction (PCR) was used to detect TCR gene rearrangement in one case of ENKTL. Results: The positive rate in 142 cases were CD2 87.9% (116/132), CD3 99.3% (141/142), CD5 33.3% (44/132), CD7 74.7% (74/99), CD4 30.2% (13/43), CD8 35.9% (14/39), CD56 92.9% (132/142), TIA-1 100% (132/132), GrB 99% (111/112), Perforin 100% (6/6), CD30 40.9% (36/88), PDGFRA 51.9% (28/54), CMYC 53.7% (29/54), Ki-67 index: 50%~90%. The results of TCR gene rearrangement was negative. Conclusion: There are the characteristic expression patterns of immunohistochemistry in ENKTL, and the abnormal expression existed.