PTEN在人脑星形细胞瘤中的表达及意义
| 作者: |
1李全,
2王广,
1冯艳萍
1 承德县中医院外一科，河北 承德 067400 2 承德医学院附属医院神经外科，河北 承德 067000 |
| 通讯: |
冯艳萍
Email: 435444923@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.07.008 |
摘要
目的：探讨磷酸酶–张力蛋白基因(phosphatase and tensin homology deleted on chromosome ten，PTEN)在正常脑组织及星形细胞瘤中的表达及其意义。方法：采用免疫组织化学法及RT-PCR方法检测正常脑组织及不同分级星形细胞瘤组织中PTEN蛋白及mRNA的表达水平，探讨并分析其与星形细胞瘤组织学分级的关系，Western印迹法检测正常脑组织及星形细胞瘤脑脊液中PTEN蛋白的表达。结果：免疫组织化学方法显示PTEN在正常脑组织中阳性率较高，为90%(9/10)，星形细胞瘤中阳性率较低，为47.5%(19/40)，且PTEN在不同的组织学分级中，阳性率不同。RT-PCR结果显示PTEN mRNA在正常脑组织中表达较高，其相对表达量为0.861±0.072，显著高于星形细胞瘤组织中的表达(0.127±0.008)，差异有统计学意义(P<0.05)，并随着组织学级别增加，其表达呈降低趋势。Western印迹法结果显示PTEN蛋白在非星形细胞瘤组脑脊液中的相对表达量为1.549±0.194，高于星形细胞瘤组脑脊液中的相对表达量(0.602±0.058)，差异具有统计学意义(P<0.05)。结论：检测PTEN基因蛋白表达有望作为评估人星形细胞瘤生物学行为和预后的参考指标。
关键词：
星形细胞瘤
磷酸酶–张力蛋白基因
Western印迹法
RT-PCR
免疫组织化学法
Expression of PTEN in human astrocytoma and its clinical significance
CorrespondingAuthor: FENG Yanping Email: 435444923@qq.com
DOI: 10.3978/j.issn.2095-6959.2017.07.008
Abstract
Objective: This study aimed to investigate the expression of phosphatase and tensin homology deleted on chromosome ten (PTEN) in human astrocytoma and its clinical significance. Methods: SP immunohistochemical and RT-PCR were used to detect the expression of PTEN in normal brain and human astrocytoma. Western blot analysis were used to detect the expression of PTEN in cerebrospinal fluid (CSF) of human astrocytoma and normal brain. Results: Immunohistochemical assay showed that positive rates of PTEN in normal brain were 90% (9/10), which were higher than that in human astrocytoma 47.5% (19/40), and negatively correlated with histological differentiating degree. RT-PCR showed that, the relative expression levels of PTEN in normal brain were 0.861±0.072, which were higher than that in human astrocytoma (0.127±0.008), the difference was statistically significant (P<0.05). Western blot showed that the relative expression levels of PTEN in CSF of normal brain were 1.549±0.194, which were higher than that in human astrocytoma (0.602±0.058) (P<0.05). Conclusion: expression of PTEN can be helpful in the prediction of the biological behavior and prognosis of human astrocytoma.