Objective: To investigate the effects of ACEI on bone metabolism and biomechanics in rats with experimental osteoporosis induced by retinoic acid. Methods: Sixty female SD rats were randomly divided into for three groups, control group (group I) and retinoic acid induced osteoporosis model group (group II) and imidapril intervention group (group III), 20 rats in each group. The rats in group II and III rats were gastro gavage by retinoic acid 80 mg/(kg·d) lasting for 21 days to establish experimental osteoporosis model. After constructing animal models, group III rats were given imidapril 10 mg/(kg·d) orally, group I and II rats received placebo-controlled, continued for 7 days. After that, the rats were killed, the body weight of rats in each group were detected . The body weight, femoral bone mineral density (BMD), and wet and dry weight, bone metabolic markers and bone biology mechanics parameters was also measured. Results: Compared with group I, the differences of the body weight, the femoral BMD, wet and dry weight, bone metabolism index of rats in group II were all statistically significant. After the intervention of ACEI imidapril, compared with group II, imidapril had increased body weight of rats, increased wet and dry weight, the difference was statistically significant (P<0.05) in group III. In group III, Femur calcium and phosphorus content increased significantly (P<0.05), the concentration of serum BGP increased significantly (P<0.05), the concentration of TRAP decreased (P<0.05), the fermoral biomechanics, including Pmax, Lmax, Mmax, bone strain and bone strength increased (P<0.05), comparing with group II. Conclusion: In an experimental osteoporosis rats model induced by retinoic acid, imidapril can elevate the rats’ body weight, increase BMD and wet and dry weight, reduce bone loss, promote bone formation and mineral deposition, increase bone biomechanical strength, and reduce the occurrence of fracture. Imidapril exerts a protective effect on osteoporosis.