Objective: To analyze the clinical distribution and antimicrobial resistance of pathogens causing diabetic foot infections in order to provide the basis for clinical rational choice of antibiotics. Methods: A total of 125 cases of diabetic foot infection were chosen from January 2011 to September 2014. The clinical data were retrospective analysed. The antimicrobial susceptibility test to isolated pathogens was performed by agar dilution method. The results were judged according to the criteria recommended by Clinical and Laboratory Standards Institute (CLSI) 2013. Results: Of 125 cases of diabetic foot infection, 132 strains were isolated, in which 62 were Gram-positive bacteria (47.0%), 55 Gram-negative bacteria (41.7%), and 15 fungi (11.4%). The results of antimicrobial susceptibility test showed that Gram-negative bacteria were highly susceptible to the carbapenem and β-lactamase inhibitor; Gram-positive bacteria were highly susceptible to the glycopeptide, carbapenem and aminoglycoside; fungus was susceptible to amphotericin B and caspofungin. Conclusion: For patients with diabetic foot infections, repeated detection of pathogens in secretions is very important. The surveillance of antimicrobial resistance should be strengthened for purpose of preventing the transmission of multidrug resistant strains.