低血清浓度下骨形成蛋白2对软骨细胞的作用
| 作者: |
1李伟豪,
2董加洪,
3许艳华,
3邓婕,
1李松
1 昆明医科大学，昆明 650500 2 丽水市中心医院口腔科，浙江 丽水 323000 3 昆明医科大学口腔医学院附属口腔医院正畸科，昆明 650101 |
| 通讯: |
李松
Email: lisong59@Sohu.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.01.025 |
| 基金: | 浙江省医药卫生科技项目, 2016KYB338 云南省科技厅–昆明医学院联合专项, 2013FZ279 国家自然科学基金, 81360163 |
摘要
目的：研究骨形成蛋白2(bone morphogenetic proteins 2，BMP-2)对SD乳鼠下颌骨髁突软骨细胞(mandibular condylar chondrocytes，MCCs)黏附和增殖作用并探索BMP-2的最佳实验浓度。方法：在低血清浓度(含2.5%胎牛血清培养基)培养条件下，分为4组(n=3)：对照组(未添加BMP-2)、BMP40组(添加40 ng/mL的BMP-2)、BMP100组(添加100 ng/mL的BMP-2)、BMP200组(添加200 ng/mL的BMP-2)。通过甲基噻唑基四唑(methyl thiazolyl tetrazolium，MTT)比色法测量8 h，12 h，1 d，3 d，5 d，7 d活细胞数并描绘生长曲线。结果：在1 d时BMP100组MTT吸光度(0.497±0.010)较对照组(0.345±0.024)、BMP40组(0.475±0.007)及BMP200组(0.471±0.013)均高，差异具有统计学意义(P<0.05)。细胞数未呈指数增长。结论：低血清浓度下，BMP-2用于MCCs的最佳实验浓度为100 ng/mL，可促进MCCs的初期黏附但增殖作用并不明显。
关键词：
髁突细胞
软骨细胞
骨形成蛋白2
Effect of bone morphogenetic proteins 2 on chondrocytes in low serum concentration
CorrespondingAuthor: LI Song Email: lisong59@Sohu.com
DOI: 10.3978/j.issn.2095-6959.2017.01.025
Abstract
Objective: To investigate the effect of bone morphogenetic proteins 2 (BMP-2) on adhesion and growth of mandibular condylar chondrocytes (MCCs) in SD rats, and explore the optimum concentration of BMP-2. Methods: The MCCs were identified and cultured in low fetal bovine serum (2.5% FBS); The cells were divided into four groups (n=3): a control group (without BMP-2), a BMP40 group (induced with 40 ng/mL BMP-2), a BMP100 (induced with 100 ng/mL BMP-2), a BMP200 (induced with 200 ng/mL BMP-2). Methyl thiazolyl tetrazolium (MTT) method was used to evaluate cell proliferation ability at 8 h, 12 h, 1 d, 3 d, 5 d, 7 d. Results: The absorbance detected by MTT assay showed that BMP100 (0.497±0.010) was significantly higher than that in control group (0.345±0.024), BMP40 group (0.475±0.007) and BMP200 group (0.471±0.013) at 1 d, the difference was statistically significant (P<0.05). However, the growth curve did not increase geometrically. Conclusion: The optimum concentration of BMP-2 is 100 ng/mL. BMP-2 can effectively promote the initial adhesion of MCCs, but the proliferation effect did not show significantly difference.