Objective: To study the clinical efficacy and safety of the comprehensive nursing intervention laparoscopic surgery for endometriosis patients. Methods: Selected 70 patients (who were diagnosed endometriosis adenomyosis and then treated by laparoscopic surgery) from March 2013 to August 2015, divide the patients into control group and observation group at random number method, 35 patients each group. Treated the control group with conventional nursing, the observation group received comprehensive nursing on basis of the control group, compared the difference between the two groups in clinical effect and adverse reaction of patients. Results: We found that the clinical effective of the observation group is significantly higher than the control group (P<0.05); the degree of pain over Ⅱ in observation group was significantly lower than the control group (P<0.05), when compared the quality of nursing, we found the observation group is higher than the control group (P<0.05), the nursing satisfactory of the patients in observation group is higher than the control group (P<0.05). Conclusion: The comprehensive nursing in the patients who were in the stage of laparoscopic surgery for endometriosis adenomyosis, which could enhance the clinical effect, decrease the untoward effect, and develop the quality of nursing, further advance the level of nursing, is worth using in clinical.