NOD-SCID小鼠移植人体胃癌实验案例分析
| 作者: |
1,2董静,
2舒翔,
3李玉红,
2唐志佼,
4闫平
1 武汉大学生命科学学院，武汉 430072 2 武汉和圣医学技术有限公司，武汉 430075 3 武汉科技大学医学院病理学系，武汉 430065 4 武汉大学基础医学院，武汉 430071 |
| 通讯: |
董静
Email: dongandjing@126.com 闫平 Email: y9621227@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.05.013 |
| 基金: | 光谷3551人才计划（第五批） 武汉市科技计划, 201260538027 |
摘要
目的：鉴定小鼠胃癌移植模型中生成的肿瘤类型及病理特征。方法：采用HE染色法对肿瘤病理组织学特征进行观察，用免疫组织化学的方法分析肿瘤中CD43、CD45RB、CD57、CK等标志物的表达及小鼠脾脏中CD43、CD57的表达以诊断肿瘤类型及来源。结果：经组织学观察，小鼠皮下肿瘤组织与人胃癌组织结构形态并不相符。经免疫组织化学分析，小鼠肿瘤抗人体的CD43、CD45RB、CD57均为阳性，提示肿瘤细胞中由大量T淋巴细胞、B淋巴细胞和NK细胞组成；但小鼠脾脏内T、B淋巴细胞和NK细胞均为阴性。结论：小鼠皮下肿瘤为淋巴瘤，来源于移植的胃癌组织中带有的淋巴组织。
关键词：
移植瘤
NOD-Scid小鼠
淋巴瘤
Case analysis of NOD-SCID mouse with patient-derived tumor xenogrraft models of gastric cancer
CorrespondingAuthor: dong jing Email: dongandjing@126.com
DOI: 10.3978/j.issn.2095-6959.2015.05.013
Abstract
Objective: The tumor type and the pathological features of tumor were identified in murine xenografts model of gastric cancer. Methods: The tumor histopathologic features was observed by HE stainings; the expression of CD43, CD45RB, CD57, CK in mouse tumor and the expression of CD43 and CD57 in mouse spleen were analyzed by immunohistochemical method to diagnose the tumor types and sources. Results: The histological features and characteristics of the mouse subcutaneous tumor were not consistent with human gastric cancer by histological observation. The CD43, CD45RB, CD57 markers were positive in mouse tumor by immunohistochemical analysis, indicating that mouse tumor cells were constituted by T lymphocytes, B lymphocytes and NK cells. But in the mouse spleen, T lymphocytes, B lymphocytes and NK cells were all negative. Conclusion: The subcutaneous tumors of the NOD-SCID mice were lymphoma and derived from lymphoid tissue in gastric cancer by transplantation.