活检组织中真菌Calcofluor White荧光染色法的改良
| 作者: |
1,2杨通,
1谭亚夏
1 广州医科大学附属第一医院呼吸疾病国家重点实验室，广州 510120 2 广州医科大学附属第四医院病理科，广州 511447 |
| 通讯: |
杨通
Email: heliantu@hotmail.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.05.015 |
| 基金: | 呼吸疾病国家重点实验室专项经费, 2007DA780158 |
摘要
目的：探索对Calcofluor White荧光染色法的改良，为活检诊断真菌感染提供一种快速、经济、对比更清晰的染色方法。方法：采用Calcofluor White M2R和苏木素、伊红染液，组合成3种染色步骤，对32例病例进行染色，筛选出适用于活检组织，并且在光镜与荧光镜下能同时观察的改良染色法步骤。结果：光镜下，组织呈现HE染色固有的形态。切换至荧光显微镜下，真菌则被显示为亮蓝色，组织背景呈黄绿色，结果清晰、醒目，对比强烈。结论：改良Calcofluor White荧光染色法能使同一张切片在光镜和荧光显微镜下同时观察，相互对照和定位，是提高真菌检出的好方法，值得在真菌的病理活检工作中广泛使用。
关键词：
真菌
荧光染色
病理活检
改良Calcofluor White
The improvement of Calcofluor White fluorescence staining method for fungi in biopsy tissues
CorrespondingAuthor: YANG Tong Email: heliantu@hotmail.com
DOI: 10.3978/j.issn.2095-6959.2015.05.015
Abstract
Objective: To explore the improvement of Calcofluor White fluorescence staining and provide a fast and economical dyeing method with striking contrast for biopsy diagnosis of fungal infection. Methods: Calcofluor White M2R, hematoxylin and eosin dyes were combined to form three different staining procedures and tostain 32 cases. The best procedure was selected, which was suitable for biopsy and for simultaneous observation of the staining under light microscope and fluorescence microscope. Results: Tissue preserved morphology of HE staining under the light microscope. Switched to the fluorescent microscope, the fungi were shown as bright blue, and the organizational background was displayed yellow to green. The results were clear, eye-catching and striking contrast. Conclusion: The modified Calcofluor White fluorescent staining method can make the same sections to be observed under light microscope and fluorescence microscope simultaneously, easy to located and mutually control. It is a good method and worthy to be widely used for detecting fungi of biopsy.