Twist 和p53 在子宫颈鳞癌及子宫颈上皮内瘤变中表达的病理意义
| 作者: |
1张鸿瑞,
2何春年
1 邢台市第三医院病理科，河北 邢台 054000 2 河北省人民医院病理科，石家庄 050051 |
| 通讯: |
张鸿瑞
Email: zhanghongrui888@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2013.02.003 |
摘要
目的:探讨Twist 基因与p53 蛋白在子宫颈鳞状细胞癌(cervical squamous cell carcinoma, SCC) 及子宫颈上皮内瘤变(cervical intraepithelial neoplasia, CIN) 中的表达及其在癌变中的作用。目的:收集宫颈SCC 组40 例, CIN 组85 例(CIN I 级30 例, II 级26 例, III 级29 例), 正常宫颈鳞状上皮(正常对照组)26 例。用自制组织芯片技术及免疫组织化学SP 法检测Twist 与p53 在SCC 组、CIN III 组、CIN II 组、CIN I 组中的表达状况, 并与正常对照组比较。结果:正常对照组、CIN I组、CIN II 组、CIN III 组及SCC 组的Twist 表达率分别为0, 10.0%, 42.3%, 48.3% 和52.5%;p53 表达率分别为0, 10.0%, 46.2%, 51.7% 和52.5%;正常对照组Twist 和p53 的表达与CIN I 组、CIN II 组、CIN III 组及SCC 组比较, 差异均有统计学意义( 均P<0.05)。SCC 组Twist 与p53 呈正相关(r=0.683, P<0.05)。结论:Twist 和p53 随着CIN 从轻到重至SCC, 表达逐渐增强, 表明两者共同参与了SCC 癌变的发生、发展过程。联合检测Twist 和p53 对CIN 分级、宫颈SCC 的病理诊断具有重要意义。
关键词：
Twist；p53；组织芯片；子宫颈上皮内瘤样病变；鳞状细胞癌
Expression of Twist and p53 in cervical squamous cell carcinoma and cervical intra-epithelial neoplasia
CorrespondingAuthor: ZHANG Hongrui Email: zhanghongrui888@126.com
DOI: 10.3978/j.issn.2095-6959.2013.02.003
Abstract
Objective: To examine the expression of Twist and p53 in cervical intra-epithelial neoplasia (CIN) and cervical invasive squamous cell carcinoma (SCC), and to explore the significance of their expression in the carcinogenesis of cervix. Methods: Twist and p53 expression in samples from 85 patients with CIN (including CIN I group, CIN II group, CIN III group), 40 patients with cervical invasive SCC (SCC group) and 26 cases of normal cervical epithelia (control group) were examined by home-made tissue chip and immunohistochemical staining (SP). Results: The positive rate for Twist expression in the control group, the CIN I group, the CIN II group, the CIN III group and the SCC group was 0, 10.0%, 42.3%, 48.3% and 53.5%, respectively; for p53 expression was 0, 10.0%, 46.2%, 51.7% and 52.5%, respectively. The expressions of Twist and p53 in the SCC group and the CIN groups were significantly higher than those in the control group (P<0.05). Twist expression was positively correlated with p53 expression in the SCC group (r=0.683, P<0.05). Conclusion: Overexpression of Twist and p53 may play an important role in the carcinogenesis of cervix development, and the detection of Twist combined with p53 may be helpful for the diagnosis of CIN and cervical SCC.