缺氧诱导因子-1α和Ki-67在乳腺浸润性导管癌中的表达及其与肿瘤微血管密度的关系
| 作者: |
1刘汝梅,
2徐启革,
2张琪
1 青岛市第八人民医院肿瘤科，山东 青岛 266300 2 淄博市第一医院病理科，山东 淄博 255200 |
| 通讯: |
徐启革
Email: xuqige_168@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.02.004 |
摘要
目的：探讨缺氧诱导因子-1α(hypoxia-inducible factor 1α，HIF-1α)和Ki-67在乳腺浸润性导管癌(invasive ductal carcinoma，IDC)中的表达及其与肿瘤微血管密度(microvessel density，MVD)的关系。方法：将82例乳腺IDC按照组织学分级分为I级组(n=11)、II级组(n=40)、III级组(n=31)；按照肿块大小分为φ1组(φmax≤2 cm，n=18)、φ2组(2 cm<φmax≤5 cm，n=50)、φ3组(φmax>5 cm，n=14)；以及根据有无淋巴结转移分为有淋巴结转移组(n=50)、无淋巴结转移组(n=32)，并随机选取同期乳腺纤维腺瘤20例作为对照组。用免疫组织化学SP法检测各分组肿瘤组织中HIF-1α，Ki-67和CD34的表达。结果：组织学分级各分组中，I级、II级与III级各分组间HIF-1α，Ki-67表达和MVD分布差异均有统计学意义(P<0.05或P<0.01)。肿块大小各分组中，φ1，φ2与φ3各分组间HIF-1α表达和MVD分布差异均有统计学意义(P<0.05或P<0.01)，但Ki-67在不同肿块大小的各分组中的差异无统计学意义(P>0.05)。有无淋巴结转移的两组间HIF-1α，Ki-67表达和MVD分布差异均有统计学意义(均P<0.01)。82例乳腺IDC中HIF-1α和Ki-67的总阳性率分别为75.6%和73.2%，MVD均值为(78.1±19.7)个/HP，与对照组相比较差异均有统计学意义(均P<0.01)。乳腺IDC中，HIF-1α(r=0.817，P<0.01)，Ki-67(r=0.656，P<0.05)与MVD均呈正相关，HIF-1α与Ki-67也呈正相关(r=0.743，P<0.05)。结论：HIF-1α，Ki-67及MVD与乳腺IDC组织学分级、肿块大小(其中Ki-67与肿块大小无相关性)和有无淋巴结转移密切相关；抑制HIF-1α的表达和/或抑制血管的生成可能在一定程度上减缓肿瘤细胞的分裂和血管转移。
关键词：
乳腺；浸润性导管癌；缺氧诱导因子-1α；Ki-67；微血管密度
Expression of hypoxia-inducible factor 1α and Ki-67 in breast invasive ductal carcinoma and its relation with microvessel density
CorrespondingAuthor: XU Qige Email: xuqige_168@126.com
DOI: 10.3978/j.issn.2095-6959.2014.02.004
Abstract
Objective: To explore the expression of hypoxia-inducible factor 1α (HIF-1α) and Ki-67 in breast invasive ductal carcinoma (IDC) and the correlation between them and microvessel density (MVD) of tumors. Methods: A total of 82 patients with breast IDC were divided into a Grade I group (n=11), a Grade II group (n=40) and a Grade III group (n=31) according to histological grade; or a φ1 group (φmax ≤2 cm, n=18), a φ2 group (2 cm < φmax ≤5 cm, n=50) and a φ3 group (φmax >5 cm, n=14) according to tumor size; or a group with metastasis of lymph nodes (n=50) and a group without metastasis of lymph nodes (n=32). Twenty cases of fibroadenoma of breast over the same period were randomly selected as a control group. Then the expression of HIF-1α, Ki-67 and CD34 were examined by immunochemistry SP method. Results: In the groups according to histological grade, there were significant statistical difference in the expression of HIF-1α, Ki-67 and distribution of MVD in these 3 groups (all P<0.05 or P<0.01). In the groups according to tumor size, there were significant statistical difference in the expression of HIF-1 αand distribution of MVD in these three groups (all P<0.05 or P<0.01), but the expression of Ki-67 was not related with the tumor size (P>0.05). There was significant statistical difference in the expression of HIF-1α, Ki-67 and distribution of MVD between the 2 groups with or without metastasis of lymph nodes (all P<0.01). The total positive rate of the expression for HIF-1α in 82 patients with breast IDC was 75.6%, for Ki-67 was 73.2%, and the average value of MVD was 78.1±19.7. There was significant statistical difference in the above indexes compared with the control group (all P<0.01). HIF-1α (r=0.817, P<0.01) and Ki-67 (r=0.656, P<0.05) were positively correlated with MVD. HIF-1α was also positively correlated with Ki-67. Conclusion: HIF-1α, Ki-67 and MVD are correlated with the breast IDC’s histological grade, tumor size (no correlation between ki-67 and tumor size) or metastasis of lymph nodes. Inhibition of the expression of HIF-1α and/or angiogenesis may slow down tumor cells division and vascular metastasis.