免疫组织化学双重染色检测肺组织p63、ck7表达在非小细胞肺癌临床诊断和转移中的应用
| 作者: |
1罗教秀,
1储兵,
2陈杰伟,
1林绮璐
1 中山市人民医院病理科，广东 中山 528403 2 中山大学肿瘤防治中心病理科，广州 510000 |
| 通讯: |
罗教秀
Email: 2029948334@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2023.221571 |
| 基金: | 中山市卫生健康局医学科研项目(2021J058)。 |
摘要
目的：探讨免疫组织化学双重染色(简称双染)检测抑癌基因p63、细胞角蛋白(cytokeratin，ck)7在非小细胞肺癌(non-small cell lung cancer，NSCLC)临床诊断和转移中的应用。方法：收集中山市人民医院2020年3月至2021年3月经外科手段活检的96例NSCLC患者的肺癌组织和癌旁组织作为研究对象。采用免疫组织化学双染法检测2种组织中p63、ck7的表达水平，并进一步分析其与NSCLC患者临床病理特征及转移的关系。结果：p63在鳞状细胞癌、有淋巴结转移、临床分期较高病例中的阳性表达率均明显高于其在腺癌、无淋巴结转移、临床分期较低病例中的阳性表达率(均P<0.05)。ck7在腺癌中的阳性表达率高于其在鳞状细胞癌中的阳性表达率(95.12% vs 21.82%，P<0.05)。在区分肺鳞状细胞癌与肺腺癌时，p63对肺鳞状细胞癌的敏感度、特异度、阳性预测值、阴性预测值及准确度分别为90.91%、65.85%、78.13%、84.38%及80.21%；ck7对肺腺癌的敏感度、特异度、阳性预测值、阴性预测值及准确度分别为95.12%、78.18%、76.47%、95.56%及85.42%。结论：p63高表达于肺鳞状细胞癌，ck7高表达于肺腺癌，二者可作为肺癌组织学分型的重要依据。
关键词：
免疫组织化学；p63；细胞角蛋白7；非小细胞肺癌；转移
Expression of p63 and ck7 in lung tissue detected by immunohistochemical double staining in the clinical diagnosis and metastasis of non-small cell lung cancer
CorrespondingAuthor: LUO Jiaoxiu Email: 2029948334@qq.com
DOI: 10.3978/j.issn.2095-6959.2023.221571
Foundation: This work was supported by the Medical Research Project of Zhongshan Municipal Health Bureau, China (2021J058).
Abstract
Objective: To investigate the application of tumor suppressor gene p63 and cytokeratin (ck)7 detected by immunohistochemical double staining in the clinical diagnosis and metastasis of non-small cell lung cancer (NSCLC).
Methods: The lung cancer tissues and adjacent tissues of 96 patients with NSCLC who underwent surgical biopsy in Zhongshan People’s Hospital from March 2020 to March 2021 were collected. The expression levels of p63 and ck7 in the 2 tissues were detected by immunohistochemical double staining, and the relationship between them and clinicopathological characteristics and metastasis of NSCLC patients was further analyzed.
Results: The positive expressions of p63 in squamous cell carcinoma, lymph node metastasis, and high clinical stage were significantly higher than those in adenocarcinoma, no lymph node metastasis, and lower clinical stage (all P<0.05). The positive expression rate of ck7 in adenocarcinoma was 95.12%, which was higher than 21.82% in squamous cell carcinoma (P<0.05). In the distinction between lung squamous cell carcinoma and lung adenocarcinoma, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of p63 for lung squamous cell carcinoma were 90.91%, 65.85%, 78.13%, 84.38%, and 80.21%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of ck7 for lung adenocarcinoma were 95.12%, 78.18%, 76.47%, 95.56%, and 85.42%, respectively.
Conclusion: p63 is highly expressed in lung squamous cell carcinoma, and ck7 is highly expressed in lung adenocarcinoma, both of them can be used as an important basis for the histological classification of lung cancer.
Keywords：
immunohistochemistry; p63; cytokeratin 7; non-small cell lung cancer; metastasis
Methods: The lung cancer tissues and adjacent tissues of 96 patients with NSCLC who underwent surgical biopsy in Zhongshan People’s Hospital from March 2020 to March 2021 were collected. The expression levels of p63 and ck7 in the 2 tissues were detected by immunohistochemical double staining, and the relationship between them and clinicopathological characteristics and metastasis of NSCLC patients was further analyzed.
Results: The positive expressions of p63 in squamous cell carcinoma, lymph node metastasis, and high clinical stage were significantly higher than those in adenocarcinoma, no lymph node metastasis, and lower clinical stage (all P<0.05). The positive expression rate of ck7 in adenocarcinoma was 95.12%, which was higher than 21.82% in squamous cell carcinoma (P<0.05). In the distinction between lung squamous cell carcinoma and lung adenocarcinoma, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of p63 for lung squamous cell carcinoma were 90.91%, 65.85%, 78.13%, 84.38%, and 80.21%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of ck7 for lung adenocarcinoma were 95.12%, 78.18%, 76.47%, 95.56%, and 85.42%, respectively.
Conclusion: p63 is highly expressed in lung squamous cell carcinoma, and ck7 is highly expressed in lung adenocarcinoma, both of them can be used as an important basis for the histological classification of lung cancer.