PARP1在结直肠癌的表达及对SW620增殖与凋亡的影响【已撤稿】
| 作者: |
1许开武,
1秦长江,
1陈志辉,
1宋新明
1 中山大学附属第一医院胃肠胰外科，中山大学胃癌诊治研究中心，广州 510080 |
| 通讯: |
宋新明
Email: songxm2010@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.06.S001 |
| 基金: | 国家自然科学基, 81172339 广东省科技计划项目, 2011B031800118 |
摘要
【本文已撤稿，请勿继续引用】
目的：探究PARP1在结直肠癌的表达情况及其与癌临床病理特征的关系，以及结肠癌细胞株SW620、LoVo细胞PARP1的表达，分析其对结直肠癌细胞株SW620增殖与凋亡的影响。方法：应用免疫组织化学检测39例结直肠癌组织及癌旁正常组织中PARP1的表达；实时荧光定量PCR(QPCR)检测结直肠癌细胞株SW620、LoVo、结肠正常黏膜细胞中PARP1的表达；转染PARP1基因shRNA表达载体后，MTT检测细胞增殖，流式细胞术检测细胞凋亡情况，QPCR、Western blotting分别检测cyclinD1及Caspase-3的基因与蛋白的表达情况。结果：PARP1在结直肠癌组织中的阳性表达率为71.8%，显著高于癌旁正常组织的20.5%(P<0.05)。PARP1在结肠癌细胞株SW620、LoVo的表达显著高于正常结肠黏膜细胞。转染PARP1基因shRNA后，PARP1水平明显减低，SW620细胞增殖能力减弱，凋亡能力增强。结论：PARP1在结直肠癌中高表达，可促进结直肠癌细胞增殖，抑制细胞凋亡。
关键词：
PARP1
结直肠癌
shRNA
细胞增殖
细胞凋亡
目的：探究PARP1在结直肠癌的表达情况及其与癌临床病理特征的关系，以及结肠癌细胞株SW620、LoVo细胞PARP1的表达，分析其对结直肠癌细胞株SW620增殖与凋亡的影响。方法：应用免疫组织化学检测39例结直肠癌组织及癌旁正常组织中PARP1的表达；实时荧光定量PCR(QPCR)检测结直肠癌细胞株SW620、LoVo、结肠正常黏膜细胞中PARP1的表达；转染PARP1基因shRNA表达载体后，MTT检测细胞增殖，流式细胞术检测细胞凋亡情况，QPCR、Western blotting分别检测cyclinD1及Caspase-3的基因与蛋白的表达情况。结果：PARP1在结直肠癌组织中的阳性表达率为71.8%，显著高于癌旁正常组织的20.5%(P<0.05)。PARP1在结肠癌细胞株SW620、LoVo的表达显著高于正常结肠黏膜细胞。转染PARP1基因shRNA后，PARP1水平明显减低，SW620细胞增殖能力减弱，凋亡能力增强。结论：PARP1在结直肠癌中高表达，可促进结直肠癌细胞增殖，抑制细胞凋亡。
Expression of PARP1 in human colorectal cancer and it relationship with tumor cell proliferation and apoptosis of SW620 cells
DOI: 10.3978/j.issn.2095-6959.2015.06.S001
Abstract
Objective: To investigate PARP1 expression in tumor and adjacent normal tissues from colorectal cancer (CRC) patients, and colon cancer lines SW620 and LoVo, and analyze the relationship between PARP1 expression and clinic pathologic features, and the effect of PARP1 expression on proliferation and apoptosis of SW620 cells. Methods: Immunohistochemistry and QPCR were respectively used to detect PARP1 expression in CRC tissues and adjacent normal intestinal mucosa, and in SW620 and LoVo cells. After transfection with PARP1-shRNA, the proliferation and apoptosis of SW620 cells were detected by MTT and flow cytometry. The gene and protein expressions of cyclinD1 and Caspase-3 were detected by QPCR and Western blotting. Results: The positive expression rate of PARP1 was 71.8% in CRC tissues, significantly higher than 20.5% in adjacent normal intestinal mucosa tissues (P<0.05). The expression levels of PARP1 in SW620, LoVo cells were significantly higher than that in normal intestinal mucosa cells. After transfection with PARP1-shRNA, PARP1 expression and cell proliferation were significantly inhibited, while tumor cell apoptosis increased. Conclusion: PARP1 expression is high in CRC, which can promote proliferation and prohibit apoptosis of tumor cells.