Objective: To investigate the effect of different sputum suction depths on blood oxygen saturation in patients with hypertensive cerebral hemorrhage and tracheotomy. Methods: From January 2020 to January 2022, 70 patients with hypertensive cerebral hemorrhage who underwent tracheotomy in Huai’an First People’s Hospital were selected. All patients underwent deep and shallow sputum suction each for 4 times on the 2nd day after tracheotomy, and the changes of pulse oxygen saturation (SpO₂) and cerebral tissue oxygen saturation (rSO₂) levels before and after suction at different sputum suction depths were compared. Results: The SpO₂ levels immediately after suction were lower than 1 min before suction in patients with deep and shallow sputum suction (both P<0.05), while the SpO₂ levels at 3, 5, and 10 min after suction were all higher than that 1 min before suction (all P<0.05). There was no statistically significant difference in SpO₂ levels at all time points between patients with deep and shallow sputum suction (all P>0.05). The rSO₂ levels at all time points after suction were higher than that 1 min before suction in patients with deep and shallow sputum suction (all P<0.05). There was no statistically significant difference in rSO₂ levels at all time points between patients with deep and shallow sputum suction (all P>0.05). The blood pressure levels of patients first increased after sputum suction and then gradually decreased to the level 1 min before sputum aspiration. The changes in blood pressures of patients with deep and shallow suction had similar patterns. Patients with deep sputum suction returned to pressure levels before suction at 5 min after suction, while those with shallow sputum suction returned to pressure levels before suction at 3 min after suction. Elevation of blood pressures within 3 min after suction was more obvious in patients with deep sputum suction compared with those with shallow sputum suction (P<0.05). Conclusion: Deep and shallow sputum suction have the same effect on patients with hypertensive cerebral hemorrhage and tracheotomy, and there is no statistically significant difference in respect of the effect on blood oxygen saturation. It is clinically recommended to implement shallow sputum suction to reduce the occurrence of adverse reactions.