Objective: To investigate the effect of skin protective film on the incidence of moisture-associated skin injury (MASD) in patients with chronic wounds treated with vacuum sealing drainage (VSD). Methods: Sixty-eight patients with chronic wounds who were treated with VSD in Burn and Plastic Surgery Department of Bozhou People’s Hospital from January 2019 to November 2021 were selected and randomly divided into a control group (n=34) and an observation group (n=34). The control group was given routine nursing, and the observation group was given skin protective film on the basis of routine nursing. Both groups were intervened for 2 weeks. When discharged, the MASD occurrence, wound treatment effect (wound cure rate, etc.) and nursing satisfaction were compared between the 2 groups. Results: Compared with the control group, the incidence of MASD in the observation group was significantly lower (P<0.05), and the occurrence time of MASD was delayed (P<0.05). Compared with the control group, the wound area reduction rate of the observation group was significantly increased  2 weeks after the intervention (P<0.05), the wound cure rate was significantly increased (P<0.05). The satisfaction rate of patients in the observation group was significantly higher than that in the control group (P<0.05). Conclusion: The use of skin protective film can effectively reduce the incidence of MASD in patients with chronic wounds undergoing VSD and promote wound healing.