Objective: To analyze the correlation between physical activity (PA) intensity and immune inflammatory status as well as quality of life in patients with Crohn’s disease (CD), so as to provide research basis for optimizing clinical decision-making. Methods: A total of 136 patients with CD in the Department of Gastroenterology of Second Hospital of Qinhuangdao from January 2018 to March 2022 were selected as a study group, and 100 physical examiners in the same period were selected as a control group. The clinical characteristics and CD4⁺ T lymphocyte percentage, CD8⁺ T lymphocyte percentage, CD4⁺/CD8⁺ T lymphocyte ratio, helper T cell (Th)17 percentage, regulatory T cell (Treg) percentage, Th17/Treg ratio in peripheral blood, and the serum immunoglobulin (Ig)A, IgG, IgM, C-reactive protein (CRP), interleukin (IL)-6, IL-10, IL-12, IL-35, and tumor necrosis factor (TNF)-α between the 2 groups were analyzed. The Chinese version of Inflammatory Bowel Disease Quality of Life Scale (IBDQ) was evaluated for the patients in the study group, and the Chinese version of International Physical Activity Questionnaire-Short (IPAQ-S) was employed to evaluate the PA intensity of patients in the study group. Results: In the study group, there were 15 cases (11.03%) at grade I, 64 cases (47.06%) at grade II and 57 cases (41.91%) at grade III of  PA intensity. The levels of CD8⁺ T lymphocyte percentage, Th17 percentage, Th17/Treg ratio in peripheral blood and the serum CRP, IL-6, IL-12, TNF-α levels of the patients in the study group were higher than those in the control group. With the increase of PA intensity of the patients in the study group, the levels of the above indexes gradually decreased, and the differences were statistically significant (all P<0.05). There were negative correlations between PA intensity and the above indexes of the patients in the study group (P<0.05). The CD4⁺ T lymphocyte percentage, CD4⁺/CD8⁺ T lymphocyte ratio, Treg percentage in the peripheral blood, and the serum IgA, IgG, IL-10, and IL-35 were higher than lower in the control group. With the increase of PA intensity of the patients in the study group, the levels of the above indicators and the total score as well as the scores of all dimensions of IBDQ increased gradually, and the differences were statistically significant (all P<0.05). There were positive correlations of PA intensity with the above indexes, the total score, the scores of all dimensions of IBDQ of the patients in the study group (all P<0.05). Conclusion: PA intensity of patients with CD is negatively correlated with immune dysfunction and up-regulation of inflammatory response, and positively correlated with their quality of life. Personalized exercise intervention for CD patients can be considered in clinic, so as to improve the curative effect and quality of life.