Objective: To investigate the correlation between high-risk human papilloma virus (HR-HPV) subtype distribution and cervical lesions in women. Methods: A total of 295 patients with cervical lesions treated in Kunshan Second People’s Hospital from January 2018 to December 2021 were selected as research objects. HPV typing was detected by polymerase chain reaction-reverse dot hybridization. According to the clinical pathological results (colposcopy biopsy), 295 patients were divided into a low-level group, a high-level group, and a cervical cancer (CC) group, and HR-HPV infection in each group was counted. Results: Among the 295 patients, 174 cases were HR-HPV positive, and the infection rate was 58.98% (174/295). The single infection accounted for 70.11% (122/174), and the multiple infection accounted for 29.89% (52/174). The infection rates of HR-HPV in the low-level group, the high-level group, and the CC group were 41.67%, 68.00%, and 88.00%, respectively, and the difference was statistically significant (all P<0.05). The HR-HPV infection rate in patients aged 41–60 years old in different cervical lesions was significantly higher than that in other age groups (P<0.05). The top three subtypes of HR-HPV infection in the low-level group were 58, 16, and 52, respectively. HR-HPV infection subtypes in the high-level group were mainly 16, 58, 52, and 33. The mainly subtypes in the CC group were mainly type 16, 18, 33. The infection rates of HR-HPV-16 and HR-HPV-18 in the CC group were significantly higher than those in the high-level group and the low-level group (P<0.05). Conclusion: The level of cervical lesions in women is related to HR-HPV subtypes and infection rate. Woman aged 41–60 years are the high incidence stage of HR-HPV infection, who should be highly valued and HR-HPV subtypes should be screened in time.