Objective: To explore the expression level of long non-coding antisense RNA FMRP translational regulator 1 antisense RNA 1 (FMR1-AS1) and its relative sense-strand RNA FMR1 in uterine cervical cancer (UCC) through integrated calculation. Methods: UCC-related high-throughput datasets were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Boxplots and Student’s t-test were conducted to compare the differences of FMR1-AS1 and FMR1 expression in UCC and normal cervical tissues. Pooled standard mean difference (SMD) was utilized to investigate the expression level of FMR1-AS1 and FMR1 in UCC. Pearson’s correlation analysis was performed to study the correlation of FMR1-AS1 and FMR1 expression in UCC. Gene set enrichment analysis (GSEA) was used to discuss the relative signaling pathway. Results: The result of pooled study revealed that FMR1-AS1 was overexpressed in UCC (SMD=0.63, 95%CI: 0.15 to 1.11). Meanwhile, the expression of FMR1 in UCC was also higher than that in normal cervical tissues via combination of 1 018 samples (678 cases of UCC and 340 cases of normal cervical tissues; SMD=0.49, 95%CI: 0.32 to 0.67). The result of Pearson’s correlation analysis demonstrated that expression of FMR1-AS1 and FMR1 was positively correlated in UCC. The results of GSEA revealed that FMR1-AS1 and FMR1 may participate the regulation of gene expression pathway. Conclusion: FMR1-AS1 and FMR1 were significantly upregulated in UCC tissues and were positively correlated. FMR1-AS1 and FMR1 may jointly facilitate tumorigenesis in UCC, which deserves further research.