Objective: To understand the drug resistance profile and genetic characteristics of IMP-producing Enterobacter cloacae strains. Methods: Two IMP-producing carbapenem-resistant Enterobacter cloacae (CRECL) were isolated from Hohhot from July 2017 to June 2021, termed FY-1 and Y-2, respectively. Species identification was performed using the VITEK-2 compact system and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial susceptibility testing was performed using the standard agar microdilution method. Whole-genome sequencing technology was applied to analyze the characteristics of drug resistance-related molecular, plasmid homology and genetic evolution. Results: FY-1 was resistant to ceftazidime/avibactam, fosfomycin and most of the β-lactam antibiotics. While intermediate to polymyxins B, and sensitive to tobramycin, gentamicin, ciprofloxacin, chloramphenicol, aztreonam and amikacin. Y-2 was resistant to all antibiotics tested except for polymyxin B, fosfomycin and amikacin . Both FY-1 and Y-2 contain one chromosome and three circular plasmids. FY-1 carried drug resistance genes fosA, ACT-7, TEM-1B and bla_IMP-1, and efflux pump system-related gene mdf(A). Y-2 carried drug-resistant genes fosA, ACT-16, aac(6’)-Ib-cr, CTX-M-3, TEM-1B and bla_IMP-8, and efflux pump system-related genes OqxA, OqxB and mdf(A). The IMP subtypes of FY-1 and Y-2 were IMP-1 and IMP-8, respectively, and both of them were located on plasmids. These two plasmids were highly homologous. The sequences of FY-1 and Y-2 were ST175 and ST114, respectively. Homology analysis suggested that strains FY-1 and Y-2 were closely related to the CRECL strains isolated from Czech Republic and Netherlands. Conclusion: The antimicrobial resistance of IMP-producing CRECL strains is severe, and their genetic relationship is high in homology. Antimicrobial resistance and molecular biology research of CRECL should be monitored in clinical.