Objective: To explore the effect of nursing intervention based on Roy adaptation model on negative emotion and bladder perfusion compliance in patients with bladder cancer perfusion chemotherapy. Methods: A total of 350 patients with bladder cancer who underwent perfusion chemotherapy in Liaoning Cancer Hospital, China Medical University Cancer Hospital from January 2021 to June 2021 were selected and randomly divided into the observation group and the control group, 175 cases in each group. The observation group was given nursing based on Roy adaptation model, and the control group was given routine nursing. The scores of Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), self-perceived burden scale (SPBS), bladder perfusion compliance and incidence of perfusion complications were compared between the two groups before and after intervention. Results: After intervention, the scores of SAS and SDS and the scores of physical factors, economic factors and emotional factors in SPBS in the observation group were significantly lower than those in the control group (P<0.05). The compliance rate of bladder perfusion in the observation group was significantly higher than that in the control group (P<0.05). The total incidence of bladder perfusion complications in the observation group was significantly lower than that in the control group (P<0.05). Conclusion: Nursing intervention based on Roy adaptation model can effectively alleviate the negative emotions of patients with bladder cancer undergoing intravesical chemotherapy, reduce their self-perceived burden, improve their bladder perfusion compliance, and reduce the incidence of bladder perfusion complications.