Objective: We aimed to investigate the prevalence of, and factors influencing, preoperative anxiety and depression in gastric cancer patients. Methods: We enrolled 53 gastric cancer patients from FujianMedical University Union Hospital, who were diagnosed by gastroscopy and would accept laparoscopic surgery. Each participant completed questionnaires to assess their anxiety and depression, coping modes, social support, type D personality and the values of Neutrophil to Lymphocyte Ratio (NLR). Results: The prevalence rate of preoperative anxiety and depression was 20.75% in the 53 gastric cancer patients. The factors were resigned dimensions of coping style, type D personality, NLR. There were no significant differences in confrontive and avoidant dimensions of coping mode and social support. Conclusion: The prevalence of preoperative anxiety and depression in gastric cancer patients is still high, and influenced by the coping mode, type D personality and NLR.