支气管肺炎患儿血清LDH3及T细胞亚群水平变化及临床意义
| 作者: |
1马兵兵,
2吴春艳,
1王花珍
1 张家口市妇幼保健院检验科，河北 张家口 075000 2 张家口市妇幼保健院儿科，河北 张家口 075000 |
| 通讯: |
马兵兵
Email: 378720965@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2021.12.011 |
| 基金: | 张家口市重点研发计划项目(1921100D)。 |
摘要
目的：研究支气管肺炎患儿血清乳酸脱氢酶3(lactate dehydrogenase 3，LDH3)及T细胞亚群水平变化及临床意义。方法：选取2019年6月至2020年6月张家口市妇幼保健院收治的细菌性支气管肺炎患儿60例作为肺炎组，按照疾病严重程度分为轻症组26例、重症组34例，另取同期同年龄段体检健康的儿童30例作为健康组。对比健康组、轻症组与重症组治疗前血清LDH3及T细胞亚群水平、肺炎组治疗前、中、后血清LDH3及T细胞亚群水平、肺炎组治疗有效与无效患儿血清LDH3及T细胞亚群水平，并对血清LDH3、T细胞亚群水平与支气管肺炎严重程度、血清LDH3与T细胞亚群水平进行Pearson相关性分析。结果：轻症组与重症组血清LDH3、CD8+水平明显高于健康组(P<0.05)，CD3+、CD4+、CD4+/CD8+水平明显低于健康组(P<0.05)，且重症组血清LDH3、CD8+水平明显高于轻症组(P<0.05)，CD3+、CD4+、CD4+/CD8+水平明显低于轻症组(P<0.05)；治疗中及治疗后肺炎患儿血清LDH3、CD8+水平明显低于治疗前(P<0.05)，CD3+、CD4+、CD4+/CD8+水平明显高于治疗前(P<0.05)，且治疗后血清LDH3、CD8+水平明显低于治疗中(P<0.05)，CD3+、CD4+、CD4+/CD8+水平明显高于治疗中(P<0.05)；肺炎组治疗有效患儿血清LDH3、CD8+水平明显低于无效患儿(P<0.05)，CD3+、CD4+、CD4+/CD8+水平明显高于无效患儿(P<0.05)；血清LDH3、CD8+水平与支气管肺炎严重程度呈正相关(P<0.05)，CD3+、CD4+、CD4+/CD8+水平与支气管肺炎严重程度呈负相关(P<0.05)；血清LDH3水平与CD8+水平呈正相关(P<0.05)，与CD3+、CD4+、CD4+/CD8+水平呈负相关(P<0.05)。结论：血清LDH3及T细胞亚群水平与患儿细菌性支气管肺炎发生发展及转归密切相关，且病情越严重，血清LDH3、CD8+水平越高，CD3+、CD4+、CD4+/CD8+水平越低。
关键词：
支气管肺炎；乳酸脱氢酶3；T细胞亚群；临床意义
Changes and clinical significance of serum LDH3 and T cell subsets in children with bronchopneumonia
CorrespondingAuthor: MA Bingbing Email: 378720965@qq.com
DOI: 10.3978/j.issn.2095-6959.2021.12.011
Foundation: This work was supported by the Key R & D Projects in Zhangjiakou, China (1921100D).
Abstract
Objective: To study the changes and clinical significance of serum lactate dehydrogenase 3 (LDH3) and T cell subsets in children with bronchopneumonia. Methods: Sixty children with bacterial bronchopneumonia treated in our hospital from June 2019 to June 2020 were selected as a pneumonia group. They were divided into a mild group (n=26) and a severe group (n=34) according to the severity of the disease. Another 30 healthy children of the same age were selected as a healthy group. The levels of serum LDH3 and T cell subsets in the healthy group, the mild group and the severe group before treatment, the levels of serum LDH3 and T cell subsets in the pneumonia group before, during and after treatment, and the levels of serum LDH3 and T cell subsets in children with effective and ineffective treatment in the pneumonia group were compared. The levels of serum LDH3, T cell subsets and the severity of bronchopneumonia, serum LDH3 and T cell subsets were analyzed by Pearson analysis. Results: The serum levels of LDH3 and CD8+ in the mild group and severe group were significantly higher than those in the healthy group (P<0.05), while the levels of CD3+, CD4+ and CD4+/CD8+ in the severe group were significantly lower than those in the healthy group (P<0.05). The serum levels of LDH3 and CD8+ in the severe group were significantly higher than those in the mild group (P<0.05), while the levels of CD3+, CD4+ and CD4+/CD8+ in the severe group were significantly lower than those in the mild group (P<0.05). The serum levels of LDH3, CD8+, CD3+, CD4+ and CD4+/CD8+ in children with pneumonia during and after treatment were significantly lower than those before treatment (P<0.05), and the levels of serum LDH3 and CD8+ after treatment were significantly lower than those during treatment (P<0.05), while the levels of CD3+, CD4+ and CD4+/CD8+ were significantly higher than those before treatment (P<0.05). In pneumonia group, the levels of serum LDH3 and CD8+ were significantly lower than those of ineffective children (P<0.05), while the levels of CD3+, CD4+ and CD4+/CD8+ were significantly higher than those of ineffective children (P<0.05). The levels of serum LDH3 and CD8+ were positively correlated with the severity of bronchopneumonia (P<0.05), while the levels of CD3+, CD4+ and CD4+/CD8+ were negatively correlated with the severity of bronchopneumonia (P<0.05). The level of serum LDH3 was positively correlated with the level of CD8+, and negatively correlated with the levels of CD3+, CD4+ and CD4+/CD8+ (P<0.05). Conclusion: The levels of serum LDH3 and T cell subsets are closely related to the occurrence, development and prognosis of bacterial bronchopneumonia. The more serious the disease is, the higher the serum levels of LDH3 and CD8+ are, and the lower the levels of CD3+, CD4+, and CD4+/CD8+ are.
Keywords：
bronchopneumonia; lactate dehydrogenase 3; T cell subsets; clinical significance