The clinicopathological features of a case of pancreatic hamartoma (PH) were retrospectively analyzed. The patient, a 62-year-old female, was admitted to the hospital because of persistent upper abdominal pain for more than 6 months without obvious inducements. Imaging examination showed that there was a solid mass in the neck of the pancreas, and tumor resection was performed for considering neuroendocrine tumor. Grossly, the tumor was a well-demarcated and solid nodular with grey-white and grayish red color on the cut section. Histologically, there was a clear boundary between the tumor and the surrounding pancreatic tissues without capsule. The tumor was composed of mature acini, ducts, and islets which disorderly distributed within the dense spindle cell stroma. Immunohistochemical staining showed that CK and CK8/18 were expressed in ductal epithelium and acini with a small number of neuroendocrine cells positive for CgA and Syn, interstitial spindle cells, expressed vimentin and CD34, but not CD117, CD99, SMA, Bcl-2, S100 and Desmin. Ki-67 proliferation index was about 2%. Histochemical staining showed that most of the elastic fibers of concentric structures around the ducts were broken or missing. PH is a rare non-neoplastic lesion of the pancreas. It is difficult to make a definite diagnosis by imaging examination before the/an operation, and histomorphology and immunohistochemistry are required for the diagnosis.