MiR-let-7b、IMP3、HMGA2在宫颈鳞癌中的表达及临床意义
| 作者: |
1,2龙玲艳,
3王晴,
1,3张蓓
1 徐州医科大学研究生学院，江苏 徐州 221000 2 徐州医科大学附属医院肿瘤科，江苏 徐州 221000 3 徐州市中心医院妇产科，江苏 徐州 221000 |
| 通讯: |
张蓓
Email: bettyzhang10@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2022.03.004 |
| 基金: | 江苏省社会发展面上项目 (BE2019636)。 |
摘要
目的：探究宫颈鳞癌组织中miR-let-7b、IMP3及HMGA2的表达情况，并探讨其相关性以及临床意义。方法：选取2016年1月至2020年7月徐州市中心医院的207例宫颈组织标本为研究对象，其中包括正常宫颈(normal cervix，NC)30例、低度鳞状上皮内病变(low-grade squamous intraepithelial lesion，LSIL)32例、高度鳞状上皮内病变(high-grade squamous intraepithelial lesion，HSIL)39例、宫颈鳞癌(squamous cancer cervix，SCC)106例。采用real-time RT-PCR法检测宫颈组织中miR-let-7b的表达水平，免疫组织化学法检测宫颈组织中IMP3、HMGA2的表达，分析miR-let-7b、IMP3、HMGA2在宫颈组织中表达的相关性以及三者与SCC患者临床病理学特征的关系。结果：MiR-let-7b在NC、LSIL、HSIL、SCC中的相对表达量呈逐渐下降的趋势(P<0.01)。IMP3在NC、LSIL、HSIL、SCC中的阳性表达率分别为3.3%、46.9%、79.5%、89.6%；HMGA2在NC、LSIL、HSIL、SCC中的阳性表达率分别为13.33%、53.13%、87.18%、88.68%。SCC、HSIL、LSIL与NC及SCC、HSIL与LSIL的IMP3及HMGA2相比，差异均有统计学意义(均P<0.05)；SCC与HSIL比较，二者的表达组间差异无统计学意义(P=0.05)。SCC患者宫颈组织中miR-let-7b、IMP3、HMGA2表达在不同年龄组的差异无统计学意义(分别P=0.691，P=0.957，P=0.132)，而三者在不同分化程度、淋巴结转移状态、FIGO分期、浸润深度的表达差异均有统计学意义(均P<0.05)。结论：SCC组织中miR-let-7b低表达，IMP3、HMGA2高表达，miR-let-7b与IMP3、HMGA2表达呈负相关，IMP3与HMGA2表达呈正相关。三者表达与SCC患者的FIGO分期、分化程度、淋巴结转移状态、浸润深度有关，联合检测miR-let-7b和IMP3、HMGA2表达可能对宫颈鳞癌的早期诊断、疾病分期、判断预后提供一定帮助。
关键词：
miR-let-7b；IMP3；HMGA2；宫颈鳞癌
Expression and clinical significance of miR-let-7b, IMP3, and HMGA2 in cervical squamous cell carcinoma
CorrespondingAuthor: ZHANG Bei Email: bettyzhang10@163.com
DOI: 10.3978/j.issn.2095-6959.2022.03.004
Foundation: This work was supported by the Social Development Project in Jiangsu Province, China (BE2019636).
Abstract
Objective: To explore the expression of miR-let-7b, IMP3, and HMGA2 in cervical squamous cell carcinoma tissues, and explore their correlation and clinical significance. Methods: A total of 207 cervical tissue specimens from Xuzhou Central Hospital from January 2016 to July 2020 were selected as the research objects, including 30 normal cervical tissue (NC), 32 low-grade squamous intraepithelial lesion (LSIL), 39 high-grade squamous intraepithelial lesion (HSIL), and 106 cases of squamous cancer cervix (SCC). Real-time RT-PCR method was used to detect the expression of miR-let-7b in cervical tissue, and immunohistochemical method was used to detect the expression of IMP3 and HMGA2 in cervical tissue. The correlation of miR-let-7b, IMP3, and HMGA2 expression in cervical tissue and the relationship between the three and the clinicopathological characteristics of patients with SCC were analyzed. Results: The relative expression of miR-let-7b in NC, LSIL, HSIL, and SCC decreased gradually (P<0.01). The positive expression rates of IMP3 in NC, LSIL, HSIL and SCC were 3.3%, 46.9%, 79.5%, 89.6%, respectively; the positive expression rates of HMGA2 in NC, LSIL, HSIL, and SCC were 13.33%, 53.13%, 87.18%, and 88.68%, respectively. Comparison of SCC, HSIL, LSIL and NC groups, and comparison of SCC, HSIL and LSIL groups, the expression of IMP3 and HMGA2 are significantly different (all P<0.05). Comparison of CSCC and HSIL groups, expression levels of both was no significant difference between groups (P=0.05). The expressions of miR-let-7b, IMP3 and HMGA2 in cervical tissues of patients with SCC were not statistically different in different age groups (P=0.691, P=0.957, P=0.132, respectively), while significant differences in the expression among the three were shown in different levels of differentiation, lymph node metastasis status, FIGO stage, and depth of invasion (all P<0.05). Conclusion: The expression of miR-let-7b is low in cervical squamous cell carcinoma, and IMP3 and HMGA2 are highly expressed. miR-let-7b has a negative correlation with IMP3 and HMGA2 expression, and IMP3 has a positive correlation with HMGA2 expression. The expressions of the three are related to the FIGO stage, differentiation degree, lymph node metastasis status and depth of invasion of cervical squamous cell carcinoma. The combined detection of miR-let-7b, IMP3 and HMGA2 expression may provide a certain help for the early diagnosis, disease staging, and prognosis of cervical squamous cell carcinoma.
Keywords：
miR-let-7b; IMP3; HMGA2; cervical squamous cell carcinoma