长链非编码RNA511对骨肉瘤Saos-2细胞增殖、迁移与凋亡的影响和机制
| 作者: |
1许常利,
2乔苏迟,
3魏华学,
1汪淼,
4汪秉轩,
1王志伟
1 海军军医大学第一附属医院骨科，上海 200433 2 海军军医大学第二附属医院905医院骨科，上海 200003 3 联勤保障部队第980医院第五派驻门诊部，石家庄 050083 4 海军军医大学第三附属医院医学影像科，上海 200433 |
| 通讯: |
王志伟
Email: doctorwangzhiwei@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2021.12.002 |
| 基金: | 国家自然科学基金(81671199)。 |
摘要
目的：探讨长链非编码RNA511(LINC00511)对人骨肉瘤细胞Saos-2增殖、凋亡与迁移的影响及其作用机制。方法：将Saos-2细胞感染LINC00511过表达的慢病毒，获得稳定感染的细胞株。LINC00511过表达后，进行CCK-8细胞增殖实验、细胞克隆形成实验、细胞凋亡实验、细胞划痕实验等观察LINC00511对Saos-2细胞增殖、凋亡以及迁移的影响。利用蛋白质印迹法检测上皮-间质转化(epithelial-mesenchymal transition，EMT)相关蛋白质E-cadherin、Vimentin和α-SMA的表达，以及PI3K/Akt信号通路中PI3K和p-Akt的蛋白质表达量，探讨LINC00511抑制骨肉瘤细胞增殖、迁移的分子调控机制。结果：Saos-2细胞中过表达LINC00511能够显著抑制骨肉瘤细胞增殖、迁移，促进细胞凋亡。Saos-2细胞中的E-cadherin蛋白表达显著升高，而Vimentin和α-SMA蛋白表达显著降低，骨肉瘤细胞的EMT受到显著抑制。此外，PI3K和p-Akt(308)蛋白表达显著降低，而p-Akt(473)蛋白表达并无明显变化，PI3K/Akt信号通路受到明显抑制。结论：LINC00511通过抑制骨肉瘤细胞的EMT以及PI3K/Akt 信号通路对骨肉瘤细胞增殖、迁移产生调控作用。LINC00511可作为骨肉瘤的潜在生物标志物和新的治疗靶点。
关键词：
长链非编码RNA511；骨肉瘤；细胞增殖；细胞迁移；细胞凋亡；机制
Effects and mechanism of long non-coding RNA511 on proliferation, migration and apoptosis of osteosarcoma Saos-2 cells
CorrespondingAuthor: WANG Zhiwei Email: doctorwangzhiwei@126.com
DOI: 10.3978/j.issn.2095-6959.2021.12.002
Foundation: This work was supported by the National Natural Science Foundation of China (81671199).
Abstract
Objective: To investigate the effects of long non-coding RNA 511 (LINC00511) on proliferation, apoptosis, and migration of human osteosarcoma cell line Saos-2 and its mechanism. Methods: Saos-2 cells were infected with LINC00511 overexpression lentivirus to obtain a stable infected cell line. After overexpression of LINC00511, the effects of LINC00511 on proliferation, apoptosis, and migration of Saos-2 cells were observed by CCK-8 cell proliferation assay, cell clone formation assay, apoptosis assay and cell scratch assay. Western blotting was used to detect the expression of EMT related proteins E-cadherin, Vimentin and α-SMA, as well as the protein expression of PI3K and p-Akt in PI3K/Akt signaling pathway, so as to explore the molecular regulatory mechanism of LINC00511 inhibiting the proliferation and migration of osteosarcoma cells. Results: Overexpression of LINC00511 in Saos-2 cells significantly inhibited the proliferation and migration of osteosarcoma cells and promoted cell apoptosis. The expression of E-cadherin protein in Saos-2 cells was significantly increased, while Vimentin and α-SMA protein expression was significantly decreased. EMT of osteosarcoma cells was significantly inhibited. In addition, the expression of PI3K and p-Akt (308) protein was significantly decreased, while p-Akt (473) protein expression was not significantly changed, and PI3K/Akt signaling pathway was significantly inhibited. Conclusion: LINC00511 regulates the proliferation and migration of osteosarcoma cells by inhibiting EMT and PI3K/Akt signaling pathway. LINC00511 can be used as a potential biomarker and a new therapeutic target for osteosarcoma.
Keywords：
long non-coding RNA 511; osteosarcoma; cell proliferation; cell apoptosis; cell migration; mechanism