小鼠变应性鼻炎发生、发展过程中MMP2和MMP9 mRNA的表达
| 作者: |
1王萌,
1顾兆伟,
1曹志伟
1 中国医科大学附属盛京医院第二耳鼻咽喉科鼻科，沈阳 110004 |
| 通讯: |
曹志伟
Email: caozw_sjhospital@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2021.09.002 |
| 基金: | 辽宁省科技厅自然科学项目 (20180550027)。 |
摘要
目的：探究基质金属蛋白酶2(matrix metalloproteinase 2，MMP2)和MMP9 mRNA在小鼠变应性鼻炎发生、发展过程中的表达，推测其作用及可能的机制。方法：序贯建立可模拟变应性鼻炎发生、发展及恢复过程的动物模型，记录各个时间节点处小鼠行为学及症状学特点并检测MMP2和MMP9 mRNA的表达。结果：致敏原激发后小鼠模型会出现打喷嚏、抓鼻等变应性鼻炎相关症状。当小鼠模型症状最强烈时，MMP2和MMP9 mRNA的表达水平明显上升；随着致敏原激发时间的延长，小鼠模型症状逐渐减轻，MMP2和MMP9 mRNA的表达水平逐渐下降。结论：在变应性鼻炎发生阶段，MMP2和MMP9 mRNA表达水平的高低与变应性鼻炎症状的严重程度相关，并可能促进局部黏膜中嗜酸性粒细胞数量的增加；在变应性鼻炎发展进程中，MMP2和MMP9 mRNA表达可能促进局部黏膜的组织重构，修复鼻黏膜的正常组织结构。
关键词：
变应性鼻炎；基质金属蛋白酶2；基质金属蛋白酶9；小鼠
mRNA expressions of MMP2 and MMP9 during the occurrence and development of allergic rhinitis in mice
CorrespondingAuthor: CAO Zhiwei Email: caozw_sjhospital@163.com
DOI: 10.3978/j.issn.2095-6959.2021.09.002
Foundation: This work was supported by the Natural Science Project of Liaoning Provincial Science and Technology Department, China (20180550027).
Abstract
Objective: To investigate the mRNA expression of matrix metalloproteinase 2 (MMP2) and MMP9 during the occurrence and development of allergic rhinitis in mice, and to speculate its role and possible mechanisms. Methods: An animal model that can simulate the occurrence, development and recovery of allergic rhinitis was sequentially established. At each time node, the behavioral characteristics of animal models were recorded, and the expression levels of MMP2 mRNA and MMP9 mRNA were detected. Results: After activating the allergen, the mice showed symptoms of allergic rhinitis such as sneezing and scratching nose. The expression levels of MMP2 mRNA and MMP9 mRNA were significantly increased when symptoms of the mice model were the strongest. With the prolongation of the sensitizing time, symptoms of mice were relieved gradually, and the expression levels of MMP2 mRNA and MMP9 mRNA were decreased gradually. Conclusion: The mRNA expression levels of MMP2 and MMP9 is related to the severity of allergic rhinitis symptoms at the onset of allergic rhinitis, and may promote the increase of eosinophils in local mucosa. During the development of allergic rhinitis, the mRNA expression of MMP2 and MMP9 may promote the tissue reconstruction of local mucosa and restore the normal tissue structure of nasal mucosa.
Keywords：
allergic rhinitis; matrix metalloproteinase 2; matrix metalloproteinase 9; mice