Objective: To explore the effect of mindfulness-based stress reduction (MBSR) on improving stigma, coping style and quality of life in patients with permanent ostomy for colorectal cancer. Methods: A total of 178 patients with colorectal cancer who received permanent colostomy in the First Affiliated Hospital of Harbin Medical University from July 2017 to December 2019 were selected by convenience sampling method, which were divided into a MBSR treatment group and a control group with 89 cases in each group by random number table method. The patients in the control group were given routine nursing intervention, and the MBSR treatment group was given MBSR intervention based on the control group. Before the intervention and 8 weeks after intervention, the social impact scale (SIS) was used to assess stigma, the simplified coping style questionnaire (SCSQ) to assess coping style, and the QLQ-C30 quality of life scale to assess quality of life. Results: There was no significant difference in SIS scores between the two groups before intervention (P>0.05). The scores of social exclusion, social isolation, economic discrimination, and intrinsic shame in the 8-week SIS scores of the two groups were lower than those before intervention (P<0.05), and the scores of the MBSR treatment group were lower than those of the control group (P<0.05). There was no statistically significant difference in the SCSQ scores between the two groups before intervention (P>0.05). The SCSQ score of the positive coping style of the two groups after 8 weeks of intervention was higher than that before intervention (P<0.05), and the SCSQ score of the negative coping style of the two groups after 8 weeks of intervention was lower than that before intervention (P<0.05). The SCSQ score of the positive coping style of the MBSR treatment group after 8 weeks of intervention was higher than that of the control group. The SCSQ score of negative coping style was lower than that of the control group (P<0.05). There was no significant difference between two groups in scores of the QLQ-C30 quality of life scale before interventions (P>0.05). The role function, emotional function, social function, cognitive function and general health scores of QLQ-C30 quality of life scale in both groups after 8 weeks intervention were higher than those before intervention (P<0.05), and the score of the MBSR treatment group was higher than that of the control group (P<0.05). Conclusion: MBSR can reduce the stigma, improve the coping style, and improve the quality of life of patients with permanent ostomy for colorectal cancer.