Objective: To explore the effect of the whole process of 3C quality nursing service mode on the disinfection and infection rate of disinfection supply center. Methods: From June 2016 to June 2017, we had 18 532 pieces of disinfection supply center equipment in Danzhou People’s Hospital without 3C quality nursing service mode as a control group. We chose 3C quality nursing service from July 2018 to July 2019. A model of disinfection supply center equipment 18 670 pieces as an observation group. The disinfection efficiency of the two groups of devices, the infection rate of the device, and the satisfaction of the staff in the nursing method were compared between the two groups. Results: The effective rate of disinfection of the observation group was higher than that of the control group (P<0.05). The incidence of device-related infection in the observation group was lower than that of the control group (P<0.05). The satisfaction of the nursing method was higher than that of the control group (P<0.05). Conclusion: The application of the 3C quality nursing service mode to the disinfection supply center can significantly improve the efficiency of instrument disinfection, reduce device-related infections, and improve satisfaction of the staff. The mode is worth promoting.