M2型肿瘤相关巨噬细胞在甲状腺乳头状癌合并桥本甲状腺炎时表达及临床意义
| 作者: |
1欧亮,
1赵志泓,
1王坤,
2史圆圆,
3袁娜娜
1 江苏大学附属医院甲乳外科，江苏 镇江 212000 2 江苏大学附属医院病理科，江苏 镇江 212000 3 江苏大学附属医院重症医学科，江苏 镇江 212000 |
| 通讯: |
赵志泓
Email: zhaozhsoochow@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.12.006 |
| 基金: | 镇江市科技创新资金(重点研发计划-社会发展)项目(SH2016030)。 |
摘要
目的：探讨M2型肿瘤相关巨噬细胞(tumor-associated macrophages，TAMs)的表达与甲状腺乳头状癌(papillary thyroid carcinoma，PTC)合并桥本甲状腺炎(Hashimoto’s thyroiditis，HT)时的相关性及临床意义。方法：收集120例甲状腺组织临床样本，包含20例结节性甲状腺肿(nodular goiter，NG)组织、20例HT组织、38例PTC和42例PTC合并HT组织。运用免疫组织化学法检测甲状腺标本组织切片中的CD68+及CD163+巨噬细胞的表达水平，并对检测结果与临床数据进行统计学分析。结果：CD68+及CD163+巨噬细胞的表达量随着HT向PTC演变而增多，且相关性分析示两者呈正相关(P<0.001)。单纯PTC CD163+细胞表达分数与CD68+细胞表达分数的比值(50/55)，明显高于PTC合并HT(76/94)。CD68+巨噬细胞的表达量与甲状腺癌的淋巴结转移情况相关(P<0.001)；CD163+巨噬细胞的表达量与甲状腺癌的肿块包膜侵犯及淋巴结转移情况相关(P=0.036，P<0.001)。结论：M2型TAMs在PTC合并HT肿瘤侵袭进展及淋巴结转移的过程中发挥了重要作用。
关键词：
甲状腺乳头状癌；桥本甲状腺炎；肿瘤相关巨噬细胞；CD163；CD68
Expression and clinical significance of M2 tumor-associated macrophages in papillary thyroid carcinoma with Hashimoto’s thyroiditis
CorrespondingAuthor: ZHAO Zhihong Email: zhaozhsoochow@163.com
DOI: 10.3978/j.issn.2095-6959.2020.12.006
Foundation: This work was supported by the Science and Technology Innovation project Foundation (Key R & D plan-social development) of Zhenjiang, China (SH2016030).
Abstract
Objective: To investigate the correlation between the expression of M2 tumor-associated macrophages (TAMs) and development of papillary thyroid carcinoma with Hashimoto’s thyroiditis and its clinical significance. Methods: A total of 120 cases of thyroid tissue were collected, including 20 nodular goiter tissues, 20 Hashimoto’s thyroiditis tissues, 38 papillary thyroid carcinomas and 42 papillary thyroid carcinomas with Hashimoto’s thyroiditis tissues. The expression of CD68+ and CD163+ macrophages in the thyroid tissue was detected by immunohistochemical method, and the results were analyzed in relation with the clinical data of the patients. Results: Immunohistochemical analysis showed that the expression of CD68+ and CD163+ macrophages increased with the development of Hashimoto’s thyroiditis to papillary thyroid cancer, and the correlation analysis showed that there was a positive correlation between them (P<0.001). The expression ratio of CD163+ cell with CD68+ cell in simple thyroid papillary carcinoma (50/55) was significantly higher than that in papillary thyroid carcinoma with Hashimoto’s thyroiditis (76/94). Clinical data showed that the expression of CD68+ macrophages was correlated with lymph node metastasis (P<0.001); the expression of CD163+ macrophages was correlated with the invasion of the capsule invasion and lymph node metastasis (P=0.036, P<0.001). Conclusion: The expression of M2 TAMs in papillary thyroid cancer with Hashimoto’s thyroiditis are related to invasion and lymphangiogenesis.
Keywords：
papillary thyroid carcinoma; Hashimoto’s thyroiditis; tumor-associated macrophages; CD163; CD68