Objective: To discuss the value of anti-Mullerian diagnostic value, inhibin B and sex hormone in children with gonadal dysplasia. Methods: A total of 150 patients who underwent serological examination in our hospital from January 2019 to June 2019 were divided into disorders of sex development (DSD) group (n=101) and Control group (n=49) according to they were diagnosed or not. Expression characteristics of serological markers in disorders of gonad development were analyzed by univariate analysis, and independent predictors of disorders of gonad development were analyzed by logistic regression. A regression equation model was established to predict the possibility of disorders of gonad development by independent predictors, and the diagnostic efficacy of the model was analyzed by using receivers’ operating characteristic curve. Results: Serum levels of AMH, INH-B, T in the DSD group was higher than that in Control group, which was statistically significant (P<0.05). Serum levels of LH, FSH, E2, PRL in the DSD group was lower than that in Control group, which was statistically significant (P<0.05). Logistic regression analysis showed that AMH, INH-B and E2 were independent predictors of disorders of gonad development. The best cut-off value of the model was 85.43, and the area under the curve was 0.943. Under this cut-off value, the sensitivity, specificity, positive predictive value, negative predictive value, and coincidence rate of this model to children with DSD were 97.02%, 75.51%, 89.09%, 92.50%, and 90.00%, respectively. Conclusion: The logistic regression equation model constructed in this study had high diagnostic efficiency for disorders of gonad development and and could be used as one of the auxiliary screening tools.