流式细胞术在噬血细胞综合征诊断中的应用进展
| 作者: |
1杨雨宇,
1杨金荣,
1曾云,
1武坤
1 昆明医科大学第一附属医院血液科，云南省血液病研究中心, 昆明 650032 |
| 通讯: |
曾云
Email: zengyun_fyy@sina.com.cn |
| DOI: | 10.3978/j.issn.2095-6959.2021.04.033 |
| 基金: | 云南省教育厅科学研究基金(2020J0172)。 |
摘要
噬血细胞性淋巴组织细胞增多症(Hemophagocytic lymphohistiocytosis，HLH)是一种以免疫系统过度激活，导致炎性细胞因子分泌过多为特征的综合征。患者通常表现为高热、细胞减少、高铁蛋白血症和肝脾肿大。其疾病过程从轻度到致命的多器官衰竭不等，该病进展迅速，且容易误诊，病死率高。对该疾病的早期认识和及时诊治有助于预防致命的后果。现行的HLH诊治中国专家共识中，对于最后2个标准[即sIL-2受体和自然杀伤(natural killer，NK)细胞活性]的测定，流式细胞术发挥着重要作用。近年来，利用流式细胞术检测潜在生物标志物为HLH的诊断开辟了新的途径。本综述将围绕流式细胞术在其中的应用展开对该疾病的一些思考。
关键词：
噬血细胞性淋巴组织细胞增多症；流式细胞术；可溶性白细胞介素-2受体；自然杀伤细胞活性；生物标志物
Advances in the application of flow cytometry in the diagnosis of hemophagocytic syndrome
CorrespondingAuthor: ZENG Yun Email: zengyun_fyy@sina.com.cn
DOI: 10.3978/j.issn.2095-6959.2021.04.033
Foundation: This work was supported by the Yunnan Provincial Department of Education Scientific Research Foundation, China (2020J0172).
Abstract
Hemophagocytic lymphohistiocytosis (HLH) is a syndrome characterized by excessive secretion of inflammatory cytokines due to excessive activation of the immune system. Patients usually present hyperpyrexia, cell loss, methemoglobinemia and hepatosplenomegaly. The course of the disease ranges from mild to fatal multiple organ failure. With high mortality, the disease progresses rapidly and is easily misdiagnosed. Early recognition and timely diagnosis and treatment of the disease are helpful to prevent fatal consequences. In the current consensus of Chinese experts on HLH diagnosis and treatment, flow cytometry plays an important role in the determination of the last two criteria [sIL-2 receptor and natural killer (NK) cell activity]. In recent years, the detection of potential biomarkers by flow cytometry has opened up a new way for the diagnosis of HLH. This review will focus on the application of flow cytometry to the disease.
Keywords：
hemophagocytic lymphohistiocytosis; flow cytometry; soluble interleukin-2 receptor; natural killer cell activity; biomarkers