沉默CIT基因可通过调控CIT相关基因的表达抑制膀胱癌细胞的进展
| 作者: |
1赵书恒,
1杨杨,
1刘赞,
1修有成
1 哈尔滨医科大学附属第一医院泌尿外科，哈尔滨 150000 |
| 通讯: |
修有成
Email: 694484688@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.11.002 |
摘要
目的：寻找Citron(CIT)基因调控膀胱癌的相关分子通路。方法：选择人膀胱癌细胞T24，沉默CIT基因，使用微阵列分析找出与正常肿瘤细胞的差异基因，对差异基因进行生物信息学分析，绘制CIT基因潜在的调控网络，对筛选出的表达水平较高的相关基因采用蛋白质印迹法进行分析验证。结果：膀胱癌细胞CIT基因可以被成功敲减，敲减后有表达上调及下调的基因，将其分为两组后进行生物信息学分析，发现CIT基因的潜在调控网络，之后选取细胞周期蛋白1(Cyclin D1，CCND1)，微小体维持蛋白3(mini chromosome maintenance 3，MCM3)，增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)，微小体维持蛋白7(mini chromosome maintenance 7，MCM7)，(cyclin dependent kinases 1，CDK1)5个基因进行蛋白质印迹法验证，发现可能存在CIT相关信号通路。结论：目的基因CIT在膀胱癌细胞上通过调控CCND1，MCM3，MCM7，CDK1基因的表达在膀胱癌的进展中发挥一定的作用。
关键词：
膀胱肿瘤；CIT基因；生物信息学；微小体维持蛋白3
Silencing CIT gene inhibits the development of bladder cancer cells by regulating CIT-related gene expression
CorrespondingAuthor: XIU Youcheng Email: 694484688@qq.com
DOI: 10.3978/j.issn.2095-6959.2020.11.002
Abstract
Objective: To explore the molecular pathway of Citron (CIT) gene regulating bladder cancer. Methods: CIT gene was silenced by the selection of human cyst cancer cell T24, and the differentially expressed genes from normal tumor cells were identified by microarray analysis. The differentially expressed genes were analyzed by bioinformatics, the potential regulatory network of CIT gene was plotted, and the screened related genes with high expression level were verified by Western blotting. Results: Bladder cancer cell CIT gene was successfully knocked out. The gene expression after knock reduction was divided into two groups after the bioinformatics analysis, showed that the potential of CIT gene regulation network, after selecting CyclinD1 (CCND1), mini chromosome maintenance 3 (MCM3), proliferating cell nuclear antigen (PCNA), mini chromosome maintenance 7 (MCM7), and cyclin dependent kinases 1 (CDK1) with Western blot verification. The underlying CIT related signaling pathway was found. Conclusion: CIT plays a role in the development of bladder cancer by regulating the expression of CCND1, MCM3, MCM7, and CDK1 genes.
Keywords：
bladder cancer; CIT gene; bioinformatics; mini chromosome maintenance 3