Objective: To analyze the application value of low-dose 64-slice CT double-phase enhanced scan in the evaluation of lymph node metastasis of gastric cancer, and to provide reference for clinical diagnosis and treatment. Methods: Forty patients with gastric cancer admitted to Liu’an Second People’s Hospital from February 2015 to May 2019 were selected for study, all patients underwent low-dose 64-slice CT double-phase enhanced scan and conventional-dose CT three-phase enhanced scan, the surgical pathological results were compared with the results of CT examination. Results: The sensitivity and specificity of low-dose 64-slice CT double-phase enhanced CT in diagnosing lymph node metastasis of gastric cancer were 83.33% and 90.00% respectively, the difference was statistically significant (P<0.05); the sensitivity of the conventional three-phase enhanced CT scan to diagnose lymph node metastasis of gastric cancer is 62.50%, and the specificity is 43.75%,the difference was statistically significant (P<0.05); the accuracy of low-dose 64-slice CT dual-phase enhanced scan for pathological staging N0-N3b was 71.43%, 60.00%, 84.62%, 75.00%, and 28.57%, the total accuracy was 67.50%. The short and long diameter of gastric cancer metastasis and non-metastasis lymph nodes were compared, and the difference was not statistically significant (P>0.05). The enhancement of metastatic lymph nodes is (48.39±5.77) HU, which is significantly higher than that of non-metastatic lymph nodes [(30.17±5.78) HU], and the difference was statistically significant (P<0.05). Conclusion: The low-dose 64-slice CT double-phase enhanced scan has significantly higher sensitivity and specificity for gastric cancer lymph node metastasis, can accurately determine the pathological stage of gastric cancer, helps improve the diagnostic coincidence rate, which is recommended to promote clinical use.