Objective: To analyze the effect of enhanced recovery after surgery (ERAS) in laparoendoscopic single site surgery-total hysterectomy (LESS-TH) perioperative period. Methods: Eighty-six patients who underwent single-port laparoscopic hysterectomy in our hospital (February 2017 to September 2018) were randomly divided into control group and observation group. The patients in the control group were treated with routine rehabilitation regimen, and the patients in the observation group were treated with ERAS. Results: The patients in the observation group were shorter in exhaust, defecation, indwelling urethral catheter and hospital stay than in the control group, and the amount of fluid rehydration on the day of operation was less than that in the control group, the difference was statistically significant (all P<0.05). The VAS score in the observation group was significantly lower than that in the control group at 2 h, 8 h, 12 h and 24 h after operation (all P<0.05). There was no significant difference in the levels of inflammatory factors and nutritional indexes between the 2 groups before operation (all P>0.05). After operation, the level of HGB, ALB in the observation group was higher than that in the control group. The level of RP, IL-6 was lower than that of control group (all P<0.05). The incidence of nausea and vomiting in the observation group was better than that in the control group, and the complication rate was lower than that in the control group (all P<0.05). Conclusion: The application of ERAS in LESS-TH perioperative period can accelerate recovery, inhibit inflammatory reaction, relieve postoperative pain, and reduce postoperative complications.