Objective: To study the clinical application value of systemic breathing training in the nursing of pulmonary nodule resection via video-assisted thoracoscopy. Methods: Eighty-two patients who underwent TV thoracoscopy for pulmonary nodule resection were randomly divided into an observation group and a control group, with 41 cases in each group. In clinical care, patients in the control group received routine nursing care, and patients in the observation group received systematic respiratory training nursing on the basis of routine care. After nursing of the two groups of patients, the respiratory function indicators (airway resistance, airway peak pressure, and respiratory work) were separately measured; and before and after the nursing intervention, the patients’ quality of life was investigated using the Short Form Health Survey-36 (SF-36). Results: After nursing, the average airway resistance, peak airway pressure, and respiratory work index values were lower in the observation group than in the control group (P<0.05). After the nursing intervention, the average score of the SF-36 quality of life scale in the observation group was higher than that in the control group (P<0.05). Conclusion: The application of systematic breathing training in the nursing of patients with pulmonary nodules by video-assisted thoracoscopy nodules can effectively improve patients’ respiratory function and quality of life.