AKIP1在胃癌中的临床意义及生物学功能
| 作者: |
1凌家生,
1颜莉芳,
1杜少雄,
1林天耀,
1谭文涛
1 惠州市中医医院消化内科，广东 惠州 516000 |
| 通讯: |
凌家生
Email: linyugdmm@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.06.002 |
摘要
目的：研究A激酶相互作用蛋白1(A kinase-interacting protein 1，AKIP1)在胃癌组织中的表达水平及临床意义，并探讨AKIP1在胃癌中的生物学功能。方法：采用qRT-PCR检测AKIP1 mRNA在50例胃癌组织和其配对的癌旁组织中的表达水平，分析AKIP1 mRNA在胃癌组织中的表达水平与患者临床病理参数的关系，采用Kaplan-Meier生存曲线分析AKIP1 mRNA表达对患者预后的影响。采用蛋白质印迹法检测4对新鲜胃癌组织和癌旁组织中AKIP1蛋白的表达。采用qRT-PCR和蛋白质印迹法检测AKIP1 mRNA、蛋白质在胃癌细胞系SGC-7901，MKN-45，MGC-803和人正常胃黏膜上皮细胞RGM-1中的表达；MGC-803经脂质体分别转染AKIP1 siRNA(si-AKIP1)和对照(si-NC)48 h后，采用MTS实验检测各组细胞增殖能力，采用Boyden实验检测各组细胞侵袭能力，采用Transwell实验检测各组细胞转移能力。结果：qRT-PCR结果显示AKIP1 mRNA在胃癌组织中的表达显著高于癌旁组织，其高表达与TNM分期和淋巴结转移有关(P<0.05)，胃癌组织中AKIP1 mRNA表达高水平的患者生存期较短。蛋白质印迹法结果显示AKIP1蛋白在胃癌组织中的表达显著高于癌旁组织。AKIP1 mRNA和蛋白在胃癌细胞系SGC-7901，MKN-45，MGC-803中的表达均显著高于人正常胃黏膜上皮细胞RGM-1，且在MGC-803细胞中的表达最高(P<0.05)；转染AKIP1 siRNA后，MGC-803细胞增殖、侵袭和转移能力均下降(P<0.05)。结论：AKIP1在胃癌组织和细胞系中均高表达，且高表达与TNM分期、淋巴结转移及不良预后相关，同时干扰AKIP1的表达抑制胃癌细胞增殖、侵袭和转移。AKIP1可以作为胃癌患者预后分子标志物及潜在治疗靶点。
关键词：
胃癌；A激酶相互作用蛋白1；临床病理参数；增殖；侵袭；转移
Clinical significance and biological function of AKIP1 in gastric cancer
CorrespondingAuthor: LING Jiashen Email: linyugdmm@sina.com
DOI: 10.3978/j.issn.2095-6959.2020.06.002
Abstract
Objective: To investigate the expression of A kinase-interacting protein 1 (AKIP1) in gastric cancer and explore its clinical significance and the biological function of AKIP1 in gastric cancer. Methods: qRT-PCR was used to detect the expression level of AKIP1 mRNA in 50 gastric cancer tissues and its matched paracancerous tissues. The relationship between the expression level of AKIP1 mRNA in gastric cancer tissues and clinic-pathological parameters was analyzed. Kaplan-Meier survival curve was used to analyze the effect of AKIP1 mRNA expression on the prognosis of patients. Western blot was used to detect the expression of AKIP1 protein in 4 pairs of fresh gastric cancer tissues and adjacent normal tissues. qRT-PCR and Western blot were used to detect the expression of AKIP1 mRNA and protein in gastric cancer cell lines SGC-7901, MKN-45, MGC-803 and human normal gastric mucosal epithelial cells RGM-1; MGC-803 was transfected with AKIP1 siRNA (si-AKIP1) and control group (si-NC) group by liposome for 48 hours, the proliferation ability of each group was detected by MTS assay, the invasion ability of each group was detected by Boyden, and the metastasis ability of each group was detected by Transwell. Results: qRT-PCR results showed that the expression of AKIP1 mRNA in gastric cancer tissues was significantly higher than that in adjacent tissues. The high expression was associated with TNM stage and lymph node metastasis (P<0.05), gastric cancer patients with high expression of AKIP1 had shorter survival. Western blot results showed that the expression of AKIP1 protein in gastric cancer tissues was significantly higher than that in adjacent tissues. The expression of AKIP1 mRNA and protein in gastric cancer cell lines SGC-7901, MKN-45 and MGC-803 was significantly higher than that in human normal gastric mucosal epithelial cells RGM-1, and the expression was highest in MGC-803 cells (P<0.05). After transfection with AKIP1 siRNA, the proliferation, invasion and metastasis ability of MGC-803 cells were decreased (P<0.05). Conclusion: AKIP1 is highly expressed in gastric cancer tissues and cell lines, and high expression is associated with TNM stage, lymph node metastasis and poor prognosis. Interfering with AKIP1 expression inhibits proliferation, invasion and metastasis of gastric cancer cells. AKIP1 can be used as a molecular marker and potential therapeutic target for gastric cancer.
Keywords：
gastric cancer; A kinase interacting protein 1; clinic-pathological parameters; proliferation; invasion