利拉鲁肽对高糖环境下体外培养的人髓核细胞凋亡的影响
| 作者: |
1姚明言,
1舒民,
2张靖,
1李志红,
3马金辉,
4景园园
1 保定市第一中心医院内分泌科，河北 保定 071000 2 河北大学附属医院心内科，河北 保定 071000 3 河北大学附属医院内分泌科，河北 保定 071000 4 保定市第一中心医院营养科，河北 保定 071000 |
| 通讯: |
李志红
Email: lizhihongmd@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.05.001 |
| 基金: | 国家自然科学基金(31701238)。 |
摘要
目的：研究不同浓度的利拉鲁肽(liraglutide，LIR)对高糖环境下体外培养的人髓核细胞(nucleus pulposus cells，NPCs)凋亡的影响。方法：培养人髓核细胞株，第三代髓核细胞随机分为对照组(CON组，NPCM细胞培养液培养)、高糖组(HG组，0.2 mol/L高糖培养液培养)、利拉鲁肽10 nmol/L干预组(LIR10组，0.2 mol/L高糖+10 nmol/L利拉鲁肽)、利拉鲁肽100 nmol/L干预组(LIR100组)：0.2 mol/L高糖+100 nmol/L利拉鲁肽、利拉鲁肽1 000 nmol/L干预组(LIR1 000组)：0.2 mol/L高糖+1 000 nmol/L利拉鲁肽。各组细胞培养48 h，CCK-8对人髓核细胞的增殖活性进行定量分析，流式细胞术及ELISA检测细胞凋亡率，细胞内活性氧(ROS)检测评估氧化应激水平。采用SPSS 22.0软件进行统计学分析。结果：与正常对照组相比，高糖组细胞增殖活性明显减低，细胞内ROS生成及凋亡率明显增加(P<0.05)。利拉鲁肽(10，100，1000 nmol/L)的干预使细胞增殖活性较高糖组明显升高，ROS的水平及细胞凋亡率明显降低(P<0.05)。在浓度为100 nmol/L时，利拉鲁肽的促进细胞增殖，抑制氧化应激及抗凋亡作用最强，各组间差异有统计学意义(P<0.05)。结论：利拉鲁肽通过抗氧化应激抑制了高糖诱导的人髓核细胞凋亡，从而发挥保护作用。
关键词：
利拉鲁肽；人髓核细胞；高糖；凋亡
Effect of liraglutide on apoptosis of human nucleus pulposus cells induced by high glucose
CorrespondingAuthor: LI Zhihong Email: lizhihongmd@163.com
DOI: 10.3978/j.issn.2095-6959.2020.05.001
Foundation: This work was supported by the National Natural Science Foundation of China (31701238).
Abstract
Objective: To evaluate the protective effect of liraglutide on apoptosis induced by high glucose in nucleus pulposus cells (NPCs). Methods: The third-generation NPCs were randomly categorized as follows: a control (CON) group (cultured in NPCM), a high glucose (HG) group (cultured in 0.2 mol/L high glucose concentration), a high glucose + liraglutide (HG+LIR) group [cultured in the high glucose (0.2 mol/L) medium containing various concentrations of liraglutide (10, 100, or 1 000 nmol/L)]. The cells were all cultured for 48 h, then cell viability was tested using cell counting kit-8; and the apoptosis rate were measured by flow cytometric analysis and ELISA; the intracellular reactive oxygen species (ROS) of the NPCs was measured using a ROS assay kit. All data were analyzed with SPSS software for Windows version 22.0. Results: Compared with the control group, cell viability decreased significantly and ROS levels, cell apoptosis rate increased significantly in the high glucose group (P<0.05). Our data demonstrated that liraglutide evidently increased cell proliferation activity and inhibited the apoptosis of NPCs induced by high glucose (P<0.05). Further analysis suggested that liraglutide suppressed ROS generation (P<0.05). The maximum effect was at 100 nmol/L of liraglutide, and the differences among different groups were statistically significant (P<0.05). Conclusion: Liraglutide could protect NPCs against high glucose-induced apoptosis by inhibiting oxidative stress.
Keywords：
apoptosis; liraglutide; nucleus pulposus cells; high glucose