The TRIM family is a class of proteins that most of them have E3 ubiquitin ligase activity. They are involved in many important biological processes, including intracellular signal transduction, protein quality control, innate immunity, autophagy, and carcinogenesis. Recent studies found that TRIM protein expression defects can lead to a variety of diseases, such as tumor, immune diseases, neuropsychiatric diseases, infectious diseases, chromosomal abnormalities and developmental diseases. TRIM3, one member of the TRIM family, is composed of the N-terminal RING domain, B-BOX domain, coiled-coil domain and C-terminal domain. It plays an important tumor suppressive function in the development and progression of various tumors, such as glioblastoma, liver cancer and colorectal cancer. TRIM3 has been considered to be a candidate tumor suppressor gene, which can inhibit tumor cell growth, invasion and metastasis, and is closely related to the clinical stage and prognosis of the tumor. The mechanism of TRIM3 function involves multiple signaling pathways such as NF-κB signaling pathway, Musashi-Notch signaling pathway and P38 signaling pathway.