Objective: To explore the clinical pathological features, diagnosis and antidiastole of myofibrils. Methods: Regression analysis is engaged to explore the clinical pathological features, treatment and prognosis of 30 diagnosed myofibrils excised by operation in Anhui Provincial Children’s Hospital in 2012–2019. Results: The participants of these 30 cases consisted of 16 males and 14 females, whose ages ranged from 42 hours to 10 years old, and the median age is 9.5 months. 9 cases of mass found at birth. The number of head-and-neck cases, appendicular cases truncal ileum-end case was 10, 8, 11 and 1 respectively. Among them, 18 were located in dermis and superficial subcutaneous layer, 8 in deep muscle, 3 in frontal bone and 1 in intestinal wall. Spindle tumor cells that presented typical band was characterized by short and circinate threadlike cells surrounding, and that rounded and polygonal cells embracing thin-wall irregular branch vessel form peripheral blood vessel cell cancer structure in the center. There were no obvious atypia and rare nuclear fission in cells. Types of immunity: cancer cells expressed Vimentin and SMA, but non-expressed S-100, Desmin, CD34, CK, CD99, CD117, β-catenin, ALK, STAT6 and GRIA2. Analysis of Ki-67 showed low growth rate (which is less than 5%). The tumors of all the patients were cut completely by surgical operation. During the 1 to 798 months of follow-up, except 3 cases which were not follow-up, the other patients survived and didn’t relapse. Conclusion: Myofibrils are rarely seen in infants and its prognosis is favorable. Correct diagnosis is inseparable from clinical, histopathology and immunohistochemical markers.