Objective: To investigate the effects of HOXB7 antisense oligonucleotides on migration and movement of oral squamous cell carcinoma. Methods: HOXB7 antisense oligonucleotides and nonsense oligonucleotides were transfected into oral squamous cell carcinoma cell line Tscca, named as AS-ODN and NS-ODN. The cells that did not transfect oligonucleotides was named as Control. Real-time PCR and Western blot were used to detect the expression of HOXB7 in cells. Cell proliferation and adhesion ability were detected by MTT. Cell scratch test was used to detect cell motility. Transwell was used to detect cell invasion and migration. Western blot was used to detect the expression levels of E-cadherin, N-cadherin, MMP-2 and MMP-9 in cells. Results: After transfection of HOXB7 antisense oligonucleotides, the expression level of HOXB7 and cell proliferation, adhesion, movement, invasion, migration decreased, the levels of N-cadherin, MMP-2 and MMP-9 in cells decreased, the level of E-cadherin protein increased, compared with the cells transfected with nonsense oligonucleotides, the difference were statistically significant(P<0.05). Conclusion: HOXB7 antisense oligonucleotides can inhibit the proliferation, adhesion, movement, invasion, migration and EMT of oral squamous cell carcinoma cells.