Objective: To investigate the association of in-hospital mortality with coexistence of hyperglycemia and elevated biomarkers of inflammation among elderly acute ischemic stroke patients. Methods: Based on the electrical medical record (EMR), we performed a retrospective study which consecutively collected data of the patients with acute ischemic stroke. After patients were grouped according clinical outcomes, demographic characteristics and clinical features were compared between groups in order to screen potential variables affecting clinical outcomes. Then, included variables were considered to be the indicators for grouping patients, with clinical outcomes as outcome variables, we performed ROC and its AUC respectively. With hospitalization time serving as time variable, survive analysis method was used to analyzed mortality risk between groups. Results: Nine hundred and twenty-six patients were enrolled in the final analysis, 377 patients of which were recorded dead during hospitalization (the dead group). After the adjustment of demographic characteristics, history of hypertension and prior stroke and elevated C-reactive protein (CRP)at admission were regarded as potential factors for the deaths of patients (P<0.05). Whereas, serum glucose level and other three inflammatory biomarkers were similar between groups. The test of ROC among risk factors indicated that the AUC of CRP was larger than others (P=0.04). The survival analysis failed demonstrate that the mortality in groups divided by CRP and hyperglycemia complicated with leukocytosis were higher than that in the control group. Conclusion: Elevated biomarkers of inflammation are risk factors for in-hospital mortality elderly patients with acute ischemic stroke.