过表达lncRNA-p21通过介导Wnt/β-catenin信号通路抑制胃癌MGC-803细胞的生长与转移
| 作者: |
1裴洪利,
2白尚星
1 沈阳市第七人民医院检验科，沈阳 110003 2 沈阳市第六人民医院检验科，沈阳 110006 |
| 通讯: |
裴洪利
Email: 1790222693@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.08.001 |
| 基金: | 沈阳市科学技术研究与发展计划项目(20147060)。 |
摘要
目的：探讨lncRNA-p21在调控胃癌MGC-803细胞生长与转移作用中的作用及Wnt/β-catenin信号通路在其中的功能。方法：在人胃癌MGC-803细胞中转染慢病毒以过表达lncRNA-p21。观察细胞形态、细胞增殖以及体内外迁移和侵袭能力，检测Wnt/β-catenin信号通路是否参与lncRNA-p21介导的抑制胃癌细胞增殖和生长作用。最后使用LiCl对Wnt/β-catenin信号通路进行验证。结果：LncRNA-p21过表达的胃癌MGC-803细胞发生了形态学的改变，表现为由纺锤状或星状形态变为较圆的低侵袭状态。LncRNA-p21在体内外均抑制了胃癌MGC-803细胞增殖和生长，体外实验表现为lncRNA-p21过表达减弱了胃癌MGC-803细胞迁移和侵袭能力。LiCl实验验证了Wnt/β-catenin信号通路介导了lncRNA-p21对胃癌MGC-803细胞增殖、迁移与侵袭的抑制作用。结论：LncRNA-p21可在体内外显著抑制胃癌MGC-803细胞的生长与转移，且该抑制作用可能通过抑制Wnt/β-catenin信号通路介导。
关键词：
lncRNA-p21；Wnt/β-catenin；信号通路；胃癌；转移
Over-expression of lncRNA-p21 inhibits the growth and metastasis of gastric cancer MGC-803 cells by mediating Wnt/beta-catenin signaling pathway
CorrespondingAuthor: PEI Hongli Email: 1790222693@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.08.001
Foundation: This work was supported by Shenyang Science and Technology Research and Development Plan Project, China (20147060).
Abstract
Objective: To investigate the role of lncRNA-p21 in regulating growth and metastasis in gastric cancer MGC-803 cells and the mediation of Wnt/β-catenin signaling pathway. Methods: Lentivirus was transfected into gastric cancer MGC-803 cells to overexpress lncRNA-p21. The cell morphology, proliferation, migration and invasion ability in vitro and in vivo were observed. Whether or not Wnt/β-catenin signaling pathway involving in the inhibition of proliferation and growth of gastric cancer cells mediated by LncRNA-p21 was detected. Finally, we used lithium chloride to validate the Wnt/β-catenin signaling pathway. Results: Over-expression of lncRNA-p21 changed morphologically from spindle or stellate to round, low invasive state in gastric cancer MGC-803 cells. LncRNA-p21 inhibited the proliferation and growth of gastric cancer MGC-803 cells in vitro and in vivo. In vitro, over-expression of lncRNA-p21 weakened the migration and invasion ability of gastric cancer MGC-803 cells. Wnt/β-catenin signaling pathway mediated the inhibitory effect of lncRNA-p21 on proliferation, migration and invasion of gastric cancer MGC-803 cells. LiCl experiments demonstrated that Wnt/β-catenin signaling pathway mediated the inhibition of LncRNA-p21 on proliferation, migration and invasion of gastric cancer MGC-803 cells. Conclusion: LncRNA-p21 can significantly inhibit the growth and metastasis of gastric cancer MGC-803 cells in vitro and in vivo, and the inhibition may be mediated by inhibiting Wnt/β-catenin signaling pathway.
Keywords：
lncRNA-p21; Wnt/β-catenin; signaling pathway; gastric cancer; metastasis