Objective: To investigate the difference of composition between primary stones and J-tube sediments, and to analyze the relationship between stone formation and physicochemical properties of urine, and provide evidence for clinical prevention and treatment of tuberculosis difficulties and prevention of stones. Methods: A total of 357 patients who underwent extubation after calculi from April 2017 to September 2018 were enrolled in Zhuzhou Central Hospital. The J-tube sedimentary stones, original stone specimens and 24 h urine biochemical components were tested. The composition between J tube sedimentary stones and the original stone, and the composition characteristics and 24-hour urine contents of different components of stone groups were compared. Results: There were differences between the J tube stone composition and the primary stone composition. Double J tube stones are mainly uric acid and ammonium urate, while primary stones are mainly calcium oxalate. The pH values of the oxalic acid calcium group, the uric acid group and the uric acid oxalic acid mixed group were all lower than the control group, and the calcium phosphate group, the ammonium magnesium phosphate group, were larger than the control group. Except for the ammonium magnesium phosphate group, the urine volume of the remaining 4 groups was less than that of the control group. Urinary calcium was higher in the oxalic acid calcium group and uric acid oxalic acid mixed group than in the control group, and the urinary phosphorus in the calcium phosphate group was lower than the control group. The urinary magnesium in the uric acid group and the ammonium magnesium phosphate group was higher than that in the control group, while the oxalic acid group was lower than the control group. The uric acid group, oxalic acid group and uric acid mixed group had higher uric acid than the control group. The calcium oxalate group mainly developed high calcium urine, as well as excessive acidified urine and high uric aciduria. The calcium phosphate group and the magnesium ammonium phosphate group were also mainly high calcium urine. The uric acid group mainly has high uric aciduria, followed by excessive acidification of urine. The uric acid mixed group mainly developed high uric aciduria, followed by oliguria, which was different from the control group. Conclusion: There is a correlation between the formation of J-tube sedimentary stones and the changes of primary stones and corresponding urine metabolism.