Objective: To investigate the regulatory effect of octreotide on intestinal immune response in rats with severe acute pancreatitis. Methods: The rats with severe acute pancreatitis were treated with octreotide to observe the protective effect of octreotide on intestinal injury. HE staining was used to observe the damage status of intestinal tissue and the expression level of inflammatory factors in blood and intestine of rats were detected. Evans blue staining and wet-dry weight ratio of intestinal tissues were used to evaluate intestinal microvascular permeability. Finally, the expression of NLRP3 in intestinal tissues was detected. Results: HE staining showed that octreotide treatment could significantly mitigated intestinal injury in rats with severe acute pancreatitis, while levels of TNF-α, IL-1β and MPO in blood of octreotide treatment group decreased significantly, and levels of TNF-a, IL-1β and myeloperoxidase (MPO) in intestine tissue were significantly inhibited. Conclusion: Octreotide can regulate intestinal immune function in rats with severe acute pancreatitis. Octreotide has protective effect on intestinal mucosal injury caused by severe acute pancreatitis.