Objective: To analyze the characteristic of precision tinnitus testing with chronic subjective tinnitus, and try to provide one-time multiple complex acoustic therapy it. Methods: A total of 68 patients with tinnitus as the first complaint in the tinnitus clinic of Xuzhou Medical University Affiliated Hospital, detailed medical history collection, precision audiology testing, tinnitus matching, tinnitus handicap inventory were detailed collected and analyzed, and given one-time sound therapy to try to initially learn about its effect. Results: Of the 68 patients, female and male rate is 35:33, with aged 12–77 years (average 46 years). The main tone of tinnitus was high frequency, and the most common tinnitus frequency was 5 993 Hz and 8 000 Hz. After the treatment, tinnitus reduction in 35 cases (51.5%), tinnitus disappeared in 7 cases (10.3%), the overall efficiency was 61.8%. There was a statistical difference between the patient’s residual inhibition test results and multiple complex sound therapy (P<0.05). Conclusion: The main tone of tinnitus in patients with chronic subjective tinnitus is concentrated at high frequency. Except the residual inhibition test, the patient’s gender, age, tinnitus main tone frequency, tinnitus main tone, tinnitus handicap inventory and other factors have nothing to do with the efficacy of the patient’s tinnitus multiple complex sound therapy. About 60% of patients achieve short-term reduction of tinnitus through multiple complex acoustic therapy. From the perspective of long-term tinnitus rehabilitation, further study is needed for long-term acoustic therapy.