Objective: To evaluate the clinical effect of minimally invasive puncture guided by color doppler ultrasound combined with ozone cavity injection in the treatment of mammary abscess. Methods: In October 2015 to October 2017, 135 patients with breast abscess from Yankuang New Mileage General Hospital were selected as the research objects. According to the law of random numbers, the patients were divided into three groups: group A was given traditional open drainage treatment, group B was given color doppler ultrasound guided minimally invasive interventional puncture with antibiotic treatment, group C was given color doppler ultrasound guided minimally invasive interventional puncture combined ozone cavity injection treatment, and the safety of the three groups of patients with clinical curative effect were compared. Results: The healing time, switching times of group C were obviously lower than those of group A, B (P<0.05); healing time, switching times of group B were obviously lower than those of group A (P<0.05), mammary fistula in group B and group C were significantly lower than that of group A (P<0.05), and the number of continued breastfeeding was significantly higher than that of group A (P<0.05). After the treatment, the VAS scores of group B and C were obviously lower than those in group A (P<0.05); the satisfaction degree of wound healing was 66.67% in group A, 88.89% in group B, 91.11% in group C, and that in group B and group C were significantly higher than that in group A (P<0.05). After the treatment, the levels of inflammatory cytokines CRP, substance P and IFN-γ of the three groups decreased significantly (P<0.05). Including inflammatory factor levels of group C was lower than that in group B and group A (P<0.05), group B was lower than that in group A (P<0.05). Conclusion: Color doppler ultrasound guided minimally invasive interventional puncture combined ozone cavity injection has better effect to treat breast abscess, which is worth popularizing in clinical application.