Objective: To explore the influencing factors of the quality of life in patients with osteoarthritis of knee (KOA), in order to provide the basis for clinical improving the prognosis and recovery of KOA disease. Methods: A total of 100 patients with KOA and 100 no-KOA people from Jan 2017 to Jul 2018 were recruited as the study objects, the information was recorded and assessed in patients with KOA and health, such as BMI classification, living conditions, the intensity of labor, genetic factors, smoking, drinking, drinking tea, eating fruits, such as physical exercise, bone density information. The information was analyzed by the logistic regression. Results: There was no significant difference in the composition of gender, age, nationality and occupation between the 2 groups (P>0.05). BMI classification, living conditions, genetic factors, smoking, drinking, drinking tea, eating fruits, physical exercise, bone mineral density distribution show significant difference in the 2 groups (P<0.05). The risk factors influencing the quality of life of KOA patients were poor living conditions, BMI, genetic factors, alcohol consumption, and physical exercise. The protective factors were tea drinking and fruit consumption. Conclusion: The high BMI, poor living conditions, abnormal genetic factors related to KOA, drinking habits and excessive physical exercise could all affect the quality of life of KOA patients. The influencing factors analysis has an important guiding significance to the clinical management, prevention and the cure for KOA disease.