Survivin 基因在胚胎性横纹肌肉瘤RD 细胞中的作用
| 作者: |
1曹娟,
1杨国城,
1张欢,
1何潇潇,
1缪秋玲,
1陈永先,
1何鹏,
2文飞球
1 深圳市儿童医院病理科，广东 深圳 518038 2 深圳市儿童医院血液肿瘤科，广东 深圳 518038 |
| 通讯: |
文飞球
Email: fwen62@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.03.005 |
| 基金: | 深圳市科技创新委员会基础研究项目(JCYJ20160429175432818)。 |
摘要
目的：探讨sur vivin基因在儿童胚胎性横纹肌肉瘤(embryonal rhabdomyosarcoma，ERMS)中的生物学作用。方法：采用sur vivin免疫组织化学检测ERMS肿瘤组织和正常骨骼肌组织的表达情况，小干扰RNA(siRNA)介导的sur vivin抑制对ERMS RD细胞凋亡的影响，原位末端标记法(TUNEL)检测RD细胞凋亡率，Western印迹法检测RD细胞caspase-3和caspase-9蛋白表达水平。结果：Sur vivin在ERMS中高表达而在正常骨骼肌组织中不表达，特异性siRNA干扰sur vivin表达导致RD细胞凋亡显著增加，sur v iv in表达的下调导致更高的凋亡率，sur v iv in表达的下调增加了caspase-3的活性，对caspase-9的活性无影响。结论：Survivin过表达可能通过抑制细胞凋亡而与ERMS发病机制有关。
关键词：
survivin；胚胎横纹肌肉瘤；凋亡；caspase
Role of survivin gene in RD cells of embryonic rhabdomyosarcoma
CorrespondingAuthor: WEN Feiqiu Email: fwen62@126.com
DOI: 10.3978/j.issn.2095-6959.2019.03.005
Foundation: This work was supported by the Basic Research Project of Shenzhen Municipal Science and Technology Innovation Committee, China (JCYJ20160429175432818).
Abstract
Objective: To investigate the biological roles of survivin in pediatric embryonal rhabdomyosarcoma (ERMS). Methods: Immunohistochemical stain with survivin-specific antibody were performed on primary ERMS tumor tissue and on normal skeletal muscle tissue. Subsequently, we explored the effect of small interfering RNA (siRNA)-mediated inhibition of survivin on the apoptosis potential in representative RD cell line. TUNEL method was used to detect the apoptosis of RD cell. The expression levels of caspase-3 and caspase-9 protein were measured by Western blot. Results: Survivin was highly expressed in ERMS primary tumor but not in normal skeletal muscle tissue. Disruption of survivin expression by survivin-specific siRNA resulted in a significant increase of apoptosis in human RD cell. Higher apoptotic rate was caused by the down-regulation of survivin expression. In addition, knockdown of survivin expression promoted the activation of caspase-3 but not caspase-9. Conclusion: Over-expression of survivin might be relevant to ERMS pathogenesis through inhibiting cell apoptosis in a caspase-dependent manner.
Keywords：
survivin; embryonal rhabdomyosarcoma; apoptosis; caspase